The study of the initiation of the flowering using Arabidopsis mutants.

使用拟南芥突变体进行开花起始的研究。

• 批准号: 13640613
• 负责人: KOMEDA Yoshibumi
• 金额: $ 1.92万
• 依托单位: The University of Tokyo (2002)Hokkaido University (2001)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13640613/
• 关键词: Arabidopsis thaliana; flowering; mutants; initiation of flowering; シロイヌナマズ; 花芽; 暗黒下; ショ糖; cop1-6; 暗黒花芽形成; もやし; AP1遺伝子; 花成遅延遺伝子; gi変異遺伝子; cop1-6変異体; 花芽形成関連遺伝子

The initial finding is that the cop1-6 mutant can develop to make flowers in complete darkness. It was initial interest that the expression or not of the genes whose functions are responsible to complete the process of the initiation of the floral development. We detected the expression of AP1, CAL, and AG genes in dark grown cop1-6 mutant by RT-PCR method, which can detect the minor amount of messages.The analysis using the double mutants of cop1-6 with co, gi and ld mutations revealed the following results : the functional point of COP1 is after that of CRY2 gene and before those of C0, GI, and LD.We also observered the effect of the sugars in the growth medium of dark culture. The results were as follows : cop1-1, cop1-4, and cop1-6 mutants could develop flowers from the concentrations of sucrose at 8%, 5% and 2%, respectively. Thus, the dark flowering may be called as induction of flowering by sucrose instead of light.We finally determined the location of the cop1-6 mutated product by the fusion of the cop1-6 mutation with the GFP=jelly fish green florescent protein. We found the location of the cop1-6 pruduct in nucleus and cytoplasm, and thus explained the phenotype of the cop1-6 mutation by this defect.

最初的发现是cop 1 -6突变体可以在完全黑暗的环境中发育开花。花发育起始过程中起决定作用的基因的表达与否一直是人们关注的问题。我们采用RT-PCR方法检测了暗生长的cop 1 -6突变体中AP 1、CAL和AG基因的表达，该方法可以检测到少量的信息。使用cop 1 -6的co、gi和ld突变的双突变体进行分析，结果显示：COP 1的功能点位于CRY 2基因之后，C 0、GI和LD的功能点之前。我们还观察了暗培养生长培养基中糖的影响。结果表明，cop 1 -1、cop 1 -4和cop 1 -6突变体分别在8%、5%和2%的蔗糖浓度下能正常开花。通过将cop 1 -6突变体与GFP=水母绿色荧光蛋白融合，最终确定了cop 1 -6突变体的定位。我们发现cop 1 -6产物在细胞核和细胞质中的定位，从而解释了cop 1 -6突变的表型。

项目成果

阿部, 高橋, 米田: "Identification of a cis-regulatory element for L1 layer-specific gene expression, which is targeted by an L1-specific homeodomain protein"The Plant Journall. 26(5). 487-494 (2001)

Abe、Takahashi、Yoneda：“L1 层特异性基因表达的顺式调控元件的鉴定，该元件是 L1 特异性同源结构域蛋白的靶点”The Plant Journall 26(5) (2001)。

Abe, M.: "PROTODERMAL FACTOR2, a gene encoding a homeodomain protein, regulates epidermal cell differentiation in the Arabidopsis shoot"The Plant Journal. (in press). (2002)

Abe, M.：“原真皮因子2，一种编码同源结构域蛋白的基因，调节拟南芥芽中的表皮细胞分化”《植物杂志》。

Taku Takahashi, Shio Matsuhara, Mitsutomo Abe, and Yoshibumi Komeda: "Disruption of a DNA topoisomerase I gene affects morphogenesis in Arabidopsis"Plant Cell. 14(9). 2085-2093 (2002)

Taku Takahashi、Shio Matsuhara、Mitsutomo Abe 和 Yoshibumi Komeda：“DNA 拓扑异构酶 I 基因的破坏影响拟南芥的形态发生”植物细胞。

高橋, 松原, 阿部, 米田: "Disruption of a DNA topoisomerase I gene affects morphogenesis in Arabidopsis"The Plant Cell. 14(9). 2085-2093 (2002)

Takahashi、Matsubara、Abe、Yoneda：“DNA 拓扑异构酶 I 基因的破坏影响拟南芥的形态发生”植物细胞 14(9) (2002)。

M. Abe, Y. Komeda, and Takahashi, T.: "Regulation of shoot epidermal cell differentiation by a pair of homeodomain proteins in Arabidopsis"Development. 130. 635-643 (2003)

M. Abe、Y. Komeda 和 Takahashi, T.：“拟南芥中一对同源结构域蛋白对芽表皮细胞分化的调节”开发。