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Molecular mechanisms for recognition and transport of proteins by a protein complex exists in peroxisomal membrane

过氧化物酶体膜中存在蛋白质复合物识别和运输蛋白质的分子机制

基本信息

批准号：
14340256
负责人：
HAYASHI Makoto
金额：
$ 9.6万
依托单位：
Okazaki National Research Institutes
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14340256/
关键词：
peroxisome protein transport lipid degradation photorespiration β-oxidation glyoxysome peroxin

项目摘要

Peroxisomal proteins are transported from the cytosol into peroxisomes by a recognition of one of the two protein targeting signals called PTS1 and PTS2. Last year, we identified two receptors (Pex5p and Pex7p) that can capture PTS 1 and PTS2, and showed protein-protein interaction between Pex5p and Pex7p, as well as Pex5p and a peroxisomal membrane protein, Pex14p. In this fiscal year, we produced transgenic plants that suppressed PEX5-and PEX7-gene expression by RNA interference technology. Analyses of these transgenic plants showed following 2 points. (1) Loss of Pex5p inhibits the import of both PTS1-and PTS2-containing proteins into peroxisomes. The transgenic plants without Pex5p showed defects in functions of both glyoxysomes and leaf peroxisomes. (2) Loss of Pex7p inhibits the import of only PTS2-containing proteins. The transgenic plants without Pex7p showed defect only in glyoxysomal function. From these observation, we concluded that Pex5p and Pex7p form a receptor-cargo complex in the cytosol, and the receptor-cargo complex is captured by the protein-protein interaction between Pex5p and the peroxisomal membrane protein, Pex14p.

过氧化物酶体蛋白通过识别称为PTS 1和PTS 2的两种蛋白质靶向信号中的一种而从胞质溶胶转运到过氧化物酶体中。去年，我们鉴定了两种可以捕获PTS 1和PTS 2的受体（Pex 5 p和Pex 7 p），并显示了Pex 5 p和Pex 7 p之间以及Pex 5 p和过氧化物酶体膜蛋白Pex 14 p之间的蛋白质-蛋白质相互作用。在本财政年度，我们通过RNA干扰技术生产了抑制PEX 5-和PEX 7-基因表达的转基因植物。对这些转基因植株的分析表明：(1)Pex 5 p的缺失抑制含PTS 1和PTS 2的蛋白质进入过氧化物酶体。不含Pex 5 p的转基因植株表现出乙醛酸酶体和叶片过氧化物酶体功能的缺陷。(2)Pex 7 p的缺失仅抑制含PTS 2蛋白的输入。不含Pex 7 p的转基因植株仅表现出乙醛酸酶体功能的缺陷。从这些观察，我们得出结论，Pex 5 p和Pex 7 p在胞质溶胶中形成受体-货物复合物，并且受体-货物复合物被Pex 5 p和过氧化物酶体膜蛋白Pex 14 p之间的蛋白质-蛋白质相互作用捕获。