DEVELOPMENT OF CLINICAL SYSTEM FOR MESENCHYMAL STEM CELL REGENERATIVE THERAPY IN RESORBED ALVEOLAR BONE

再生牙槽骨间充质干细胞再生治疗临床系统的开发

• 批准号: 16209057
• 负责人: HOSOKAWA Ryuji
• 金额: $ 30.53万
• 依托单位: Kyushu Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16209057/
• 关键词: REGENARATIVE DENTISTRY; BONE REGENERATION; DENTISTRY; GALECTINE; CHROMOGRANIN A; ORAL HEALTH RELATED QOL; OHIP; PARAFUNCTION; 幹細胞; 再生医学; トランスレーショナルリサーチ; トランスレーションリサーチ; 再生医療; 歯科用インプラント; 間葉系細胞; PRP

We have studied on several aspects of clinical application system development for regenerative therapy in dentistry. The summary of the results is as follows ;1) We evaluate the reliability and validity of a Japanese short version of the Oral Health Impact Profile (OHIP-JP16) for regenerative therapy in dentistry. The results showed that internal consistency and reproducibility demonstrated quite a good reliability. As a result of having examined for behaviormetrics study, OHIP-JP16 showed good measurement properties and might be appropriate for use in the clinical settings.2) We investigated the effects of galectin-9 on osteoblast proliferation and its signaling mechanisms. Galectin-9-induced proliferation of the obtained osteoblasts in a dose-dependent manner, whereas galectin-1, -3, and -4 did not. Galectin-9-induced phosphorylation of c-Src and subsequent ERK1/ERK2 in the osteoblasts. Galectin-9-induced clustering of lipid rafts detected by cholera toxin B (CUB; binding the raft-resident ganglioside GM1) using confocal microscopy. These results suggest that galectin-9, but not other galectins, induced proliferation of human osteoblasts through clustering lipid rafts on membrane and subsequent phosphorylation of the c-Src/ERK signaling pathway.3) Bruxism and parafunctions are potential risk factors for implant / regenerative therapy, therefore we investigated the relationship between bruxism behavior and a salivary stress biomarker level There was a positive correlation between self-reported bruxism and self.reported jaw functional limitation. The CgA level was significantly negative in correlation with bruxism behavior. Our results suggest that daytime physiological stress level is significantly negative in correlation with sleep bruxism behavior.

我们对牙科再生治疗临床应用系统开发的几个方面进行了研究。研究结果总结如下：1)我们评估了日语版口腔健康影响量表(OHIP-JP16)在牙科再生治疗中的可靠性和有效性。结果表明，该量表具有较好的内部一致性和重复性。作为行为计量学研究的结果，OHIP-JP16显示出良好的测量性能，可能适合用于临床设置。2)我们研究了Galectin-9对成骨细胞增殖的影响及其信号机制。Galectin-9以剂量依赖的方式诱导成骨细胞增殖，而Galectin-1、-3和-4不能。Galectin-9诱导成骨细胞c-Src和随后的ERK1/ERK2的磷酸化。用共聚焦显微镜检测由霍乱毒素B(CUB；结合木筏驻留的神经节苷脂GM1)检测到的Galectin-9诱导的脂筏聚集。这些结果表明，Galectin-9，而不是其他Galectin，通过聚集在膜上的脂筏和随后的c-Src/ERK信号通路的磷酸化来诱导人成骨细胞的增殖。3)磨牙症和功能不全是种植/再生治疗的潜在危险因素，因此我们研究了磨牙症行为与唾液应激生物标志物水平的关系。CGA水平与磨牙症行为呈显著负相关。我们的结果表明，白天的生理应激水平与睡眠磨牙症行为呈显著负相关。

项目成果

Effects of Sleep Bruxism Behavior on Salivary Physiological Stress Biomarker Level

睡眠磨牙行为对唾液生理应激生物标志物水平的影响

• 作者: MAKINO M;et. al.
• 通讯作者: et. al.

Interaction of galectin-9 with lipid rafts induces osteoblast proliferation through the c-Src/ERK signaling pathway

• DOI: 10.1359/jbmr.071008
• 发表时间: 2008-02-01
• 期刊: JOURNAL OF BONE AND MINERAL RESEARCH
• 影响因子: 6.2
• 作者: Tanikawa, Rena;Tanikawa, Takahisa;Tanaka, Yoshiya
• 通讯作者: Tanaka, Yoshiya

ミニマルインターベンションインプラント患者中心の治療戦略ガイド

以患者为中心的微介入种植治疗策略指南

• 发表时间: 2007
• 作者: 細川 隆司;他
• 通讯作者: 他

Effects of Sleep Bruxism Behavior on Salivary Physiological Stress Biomarker

睡眠磨牙行为对唾液生理应激生物标志物的影响

• 期刊: Int.Journal of Prosthodontics (In press)
• 作者: Makino;M.;et. al.
• 通讯作者: et. al.

Small GTPase Rho Signaling Regulates Bone Metabolism

小 GTPase Rho 信号传导调节骨代谢

• 发表时间: 2007
• 作者: TANIKAWA R;et. al.
• 通讯作者: et. al.