ALVEOLAR BONE REGENERATION BY USING MESENCHYMAL STEM CELL AND PLATELET-RICH PLASMA

本研究的目的是确定富血小板血浆（PRP）和间充质干细胞（MSC）的结合是否会加速犬牙槽缺损模型的新骨形成。在beagle犬无牙区两侧各制备3个骨缺损，尺寸为3.75 x 7.0mm（直径x深）。然后用以下四种材料中的一种填充缺陷：1)PRP+MSC, 2) PRP, 3)无血小板血浆（PPP）和4)血凝块（对照）。术后2周采用组织学和形态学方法评估新骨形成情况。结果表明，PRP+MSC和PRP填充缺损的骨再生面积较大。在接下来的实验中，在每个无牙区颊侧制造两个骨缺损。然后在两个缺陷中分别放置钛牙种植体。然后用以下四种材料中的一种填充缺损：1)血凝块（对照），2)PRP, 3) AB移植物和4)带PRP的自体骨（AB）移植物。术后10周，采用组织学和组织形态学方法评估颊缺损处的新骨形成情况。组织学上，所有缺损均被新生骨填充，但有一定差异。虽然所有暴露的螺钉都被AB和PRP移植的新骨覆盖，但对照组和PRP单独位置的暴露螺钉没有完全覆盖。对照组、PRP、AB植骨和AB植骨与PRP的再生骨接触率分别为24.1±20.8%、59.7±8.8%、39.2±3.5%、41.1±13.5%，PRP部位骨与种植体的接触率显著高于对照组（p<0.05）。

The purpose of this study was to determine whether a combination of platelet-rich plasma (PRP) and mesenchymal stem cells (MSC) would accelerate new bone formation in a canine alveolar defect model. Three bone defects, measuring 3.75 x 7.0mm (diameter x deep) were prepared on each side of the edentulous areas in beagle dogs. The defects were then filled with one of the following four materials : 1) PRP+MSC, 2) PRP, 3) platelet-poor plasma (PPP), and 4) blood clot (control). New bone formation was assessed by histologic and morphometric methods 2 weeks after surgery. The results showed that a larger area of regenerated bone was seen in the defects that were filled with PRP+MSC and PRP. In the next experiment, two bone defects were created at the buccal side of each edentulous area. Titanium dental implants were then placed in each of two defects. Then the defects were filled with one of the following four materials : 1) blood clot (Controls), 2) PRP, 3) AB graft and 4) autogeneous bone (AB) graft with PRP. Ten weeks after surgery, new bone formation at the buccal defects was assessed using histological and histomorphometrical methods. Histologically, all defects were filled with newly formed bone with some variation. Although all exposed screws were covered with newly formed bone at the AB graft and the AB graft with PRP, exposed screws in the Control and PRP alone sites were not fully covered. The regenerated bone contact ratio of Controls, PRP, AB graft and AB graft with PRP were 24.1±20.8%, 59.7±8.8%, 39.2±3.5%, 41.1±13.5%, respectively Bone-implant contact was significantly higher (p<0.05) at the PRP site compared with the control site.