ALVEOLAR BONE REGENERATION BY USING MESENCHYMAL STEM CELL AND PLATELET-RICH PLASMA

使用间充质干细胞和富含血小板的血浆进行牙槽骨再生

• 批准号: 14571846
• 负责人: HOSOKAWA Ryuji
• 金额: $ 2.56万
• 依托单位: KYUSHU DENTAL COLLEGE (2003)Hiroshima University (2002)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571846/
• 关键词: mesenchymal stem cell; dental implants; PRP; 骨再生; 顎骨再生; 骨髄幹細胞; ビーグル犬; 歯科インプラント; FGF-2; 組織形態計測; 骨接触率

The purpose of this study was to determine whether a combination of platelet-rich plasma (PRP) and mesenchymal stem cells (MSC) would accelerate new bone formation in a canine alveolar defect model. Three bone defects, measuring 3.75 x 7.0mm (diameter x deep) were prepared on each side of the edentulous areas in beagle dogs. The defects were then filled with one of the following four materials : 1) PRP+MSC, 2) PRP, 3) platelet-poor plasma (PPP), and 4) blood clot (control). New bone formation was assessed by histologic and morphometric methods 2 weeks after surgery. The results showed that a larger area of regenerated bone was seen in the defects that were filled with PRP+MSC and PRP. In the next experiment, two bone defects were created at the buccal side of each edentulous area. Titanium dental implants were then placed in each of two defects. Then the defects were filled with one of the following four materials : 1) blood clot (Controls), 2) PRP, 3) AB graft and 4) autogeneous bone (AB) graft with PRP. Ten weeks after surgery, new bone formation at the buccal defects was assessed using histological and histomorphometrical methods. Histologically, all defects were filled with newly formed bone with some variation. Although all exposed screws were covered with newly formed bone at the AB graft and the AB graft with PRP, exposed screws in the Control and PRP alone sites were not fully covered. The regenerated bone contact ratio of Controls, PRP, AB graft and AB graft with PRP were 24.1±20.8%, 59.7±8.8%, 39.2±3.5%, 41.1±13.5%, respectively Bone-implant contact was significantly higher (p<0.05) at the PRP site compared with the control site.

本研究的目的是确定富含血小板血浆（PRP）和间充质干细胞（MSC）的组合是否会加速犬牙槽骨缺损模型中新骨的形成。在比格犬无牙颌区域的每一侧制备三个尺寸为 3.75 x 7.0mm（直径 x 深度）的骨缺损。然后用以下四种材料之一填充缺陷：1) PRP+MSC、2) PRP、3) 贫血小板血浆 (PPP) 和 4) 血凝块（对照）。术后2周通过组织学和形态测量方法评估新骨形成。结果显示，用PRP+MSC和PRP填充的缺损处可见较大面积的再生骨。在下一个实验中，在每个无牙区域的颊侧产生两个骨缺损。然后将钛牙种植体分别植入两个缺损处。然后用以下四种材料之一填充缺损：1）血凝块（对照），2）PRP，3）AB 移植物和 4）带有 PRP 的自体骨（AB）移植物。手术后十周，使用组织学和组织形态测量方法评估颊缺损处的新骨形成。从组织学上看，所有缺损都被新形成的骨填充，但存在一些变化。尽管所有暴露的螺钉在 AB 移植物处都被新形成的骨覆盖，并且 AB 移植物具有 PRP，但对照和单独 PRP 部位的暴露螺钉并未完全覆盖。对照组、PRP、AB移植物和带PRP的AB移植物的再生骨接触率分别为24.1±20.8%、59.7±8.8%、39.2±3.5%、41.1±13.5%，与对照部位相比，PRP部位的骨-种植体接触显着更高（p<0.05）。

项目成果

インプラント周囲骨欠損に対する多血小板血漿の骨再生促進効果に関する実験的研究

富血小板血浆促进种植体周围骨缺损骨再生作用的实验研究

• 发表时间: 2003
• 期刊: 広大歯誌 35
• 作者: J.Kitagawa;竹腰利英
• 通讯作者: 竹腰利英

PRP(plate-rich-plasma)の臨床応用

PRP（富板血浆）的临床应用

• 发表时间: 2002
• 期刊: 広大歯誌 34
• 作者: Y.Kajii;細川隆司
• 通讯作者: 細川隆司

PRP(plate-rich-plasma)による組織再生促進のメカニズム-臨床への展開と今後の課題-

PRP（富板血浆）促进组织再生的机制 - 临床进展和未来挑战 -

• 发表时间: 2002
• 期刊: 歯界展望 100
• 作者: Y.Kakizaki;細川隆司 他
• 通讯作者: 細川隆司 他

AN EXPERIMENTAL STUDY ON ACCELERATION OF BONE REGENERATION WITH PLATELET-RICH PLASMA OF BONE DEFECT AROUND DENTAL IMPLANT

牙种植体周围骨缺损富血小板血浆加速骨再生的实验研究

• 发表时间: 2003
• 期刊: J Hiroshima Univ.Dent.Soc. 35
• 作者: TAKEKOSHI;T
• 通讯作者: T

インプラント補綴の最新エビデンス

种植修复体的最新证据

• 发表时间: 2004
• 作者: 細川隆司;渡邊文彦 編
• 通讯作者: 渡邊文彦 編