ALVEOLAR BONE REGENERATION BY USING MESENCHYMAL STEM CELL AND PLATELET-RICH PLASMA

本研究的目的是确定富血小板血浆（PRP）和间充质干细胞（MSC）的组合是否会加速犬牙槽骨缺损模型中新骨的形成。在比格犬无牙区的每侧制备3个骨缺损，尺寸为3.75 x 7.0 mm（直径x深）。然后用以下四种材料中的一种填充缺损：1）PRP+MSC，2）PRP，3）贫血小板血浆（PPP）和4）血凝块（对照）。术后2周通过组织学和形态学方法评估新骨形成。结果显示，在PRP+MSC和PRP填充的缺损中观察到更大面积的再生骨。在接下来的实验中，在每个无牙区的颊侧创建两个骨缺损。然后将钛牙科种植体放置在两个缺损中的每一个中。然后用以下四种材料之一填充缺损：1）血凝块（对照），2）PRP，3）AB移植物和4）含PRP的自体骨（AB）移植物。术后10周，采用组织学和组织形态计量学方法评估颊部缺损处的新骨形成。组织学上，所有缺损均填充有新生骨，但存在一定差异。尽管在AB移植物和含PRP的AB移植物处所有暴露的螺钉均被新形成的骨覆盖，但对照部位和仅含PRP部位的暴露螺钉未被完全覆盖。对照、PRP、AB移植物和AB移植物与PRP的再生骨接触率分别为24.1± 20.8%、59.7± 8.8%、39.2± 3.5%、41.1± 13.5%。与对照部位相比，PRP部位的骨-植入物接触显著更高（p<0.05）。

The purpose of this study was to determine whether a combination of platelet-rich plasma (PRP) and mesenchymal stem cells (MSC) would accelerate new bone formation in a canine alveolar defect model. Three bone defects, measuring 3.75 x 7.0mm (diameter x deep) were prepared on each side of the edentulous areas in beagle dogs. The defects were then filled with one of the following four materials : 1) PRP+MSC, 2) PRP, 3) platelet-poor plasma (PPP), and 4) blood clot (control). New bone formation was assessed by histologic and morphometric methods 2 weeks after surgery. The results showed that a larger area of regenerated bone was seen in the defects that were filled with PRP+MSC and PRP. In the next experiment, two bone defects were created at the buccal side of each edentulous area. Titanium dental implants were then placed in each of two defects. Then the defects were filled with one of the following four materials : 1) blood clot (Controls), 2) PRP, 3) AB graft and 4) autogeneous bone (AB) graft with PRP. Ten weeks after surgery, new bone formation at the buccal defects was assessed using histological and histomorphometrical methods. Histologically, all defects were filled with newly formed bone with some variation. Although all exposed screws were covered with newly formed bone at the AB graft and the AB graft with PRP, exposed screws in the Control and PRP alone sites were not fully covered. The regenerated bone contact ratio of Controls, PRP, AB graft and AB graft with PRP were 24.1±20.8%, 59.7±8.8%, 39.2±3.5%, 41.1±13.5%, respectively Bone-implant contact was significantly higher (p<0.05) at the PRP site compared with the control site.