Collection and Investigation into Basic Evolutionary Information by Orrsite Collaboration

在2004年至2007年的资助期内，我们共访问了16个国家的67所大学和研究机构，以完成我们的调查目的。由于DDBJ与美国的GenBank和欧洲的EMBL Bank组织了国际核苷酸序列数据库合作（INSDC），我们频繁访问这两个研究机构是至关重要和富有成效的，导致出版的条目数量在四年内从3500万增加到8000万（或400到820亿个碱基）。已发表的条目包括许多物种的表达序列和具有生物信息的全基因组序列，因此是生物学和相关领域研究不可或缺的。我们还缝制了GIB（细菌基因组数据库）、GTOP（蛋白质结构预测数据库）和CIBEX（基因表达数据库）等指定数据库。这些数据库的内容和用户数量都因本赠款支助的各种国际访问而得到丰富。一些国际访问还为开展人类基因、水稻基因和小鼠基因的数据注释做出了贡献。注释结果已发表在各自的数据库和期刊上。例如，我们已经表明，人类基因组中的基因总数约为35000个，这比以前估计的要大得多。最近，我们参与了基因组序列数据的国际标准化，发布了真核生物、原核生物、病毒、质粒等基因组序列注释指南。访问成果在《Nature》、《Nature Biotechnology》、《Nature Genetics》、《Science》、《Proc. Natl》等国际知名学术期刊上发表。学会科学。美国，基因组研究和核酸研究（见出版物列表）。

In the term of the present grant from 2004 to 2007 we visited 67 universities and research institutes in 16 countries in total to fulfill our investigation purposes. Since we DDBJ organize the International Nucleotide Sequence Database Collaboration (INSDC) with GenBank in USA and EMBL Bank in Europe, our frequent visits to these two research organizations were crucial and fruitful, resulted in a steep increase in the number of published entries from 35 to 80 million sequences (or 40 to 82 billon bases) in the four years. The published entries include expressed sequences and the whole genome sequence with biological information for many species and thus indispensable for research in biology and related areas. We have also sewed specified databases such as GIB (bacterial genome database), GTOP (protein-structure prediction database) and CIBEX (gene expression database). These databases have been enriched in the contents and the number of users because of various international visits supported by the present grant.Some of the international visits have also contributed to carrying out data annotations of human genes, rice genes and mouse genes. The results of the annotations have been published in their respective databases and journals. For example, we have shown that the total number of genes in the human genome is about 35, 000 that is much larger than previously estimated.Recently, we participated in the international standardization of genome sequence data and published the guidelines for annotating genome sequences of eukaryotes, prokaryotes, viruses, plasmids and others.The outcomes of the international visits have been published in internationally well-known academic journals such as Nature, Nature Biotechnology, Nature Genetics, Science, Proc. Natl. Acad. Sci. USA, Genome Research and Nucleic Acids Research (see the list of publications).