Elektrospray-Ionisation (ESI) mass spectrometry system for protein analyses

用于蛋白质分析的电喷雾电离 (ESI) 质谱系统

• 批准号: 450267567
• 金额: --
• 依托单位: Gottfried Wilhelm Leibniz Universität Hannover
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/450267567?language=en
• 关键词: Elektrospray; Ionisation; ESI; mass; spectrometry

Research of the Plant Proteomics department of the Faculty of Natural Sciences of Leibniz Universität Hannover is focused on molecular processes of plants on the level of proteins and proteomes. Protein mass spectrometry is at the very center of our projects. In addition, our department is engaged in analyzing protein fractions from various other Institutes of the Herrenhausen campus of Leibniz Universität Hannover. Currently, our department is running two mass spectrometry systems for protein analyses which are used to capacity. Mass spectrometry systems much evolved during the last decade. The requested new mass spectrometry system would significantly enhance the research capabilities of our department and allows investigating molecular functions of plants in a depth currently not possible for us.The plant proteomics department is especially interested in exploring the energy biology of plants, particularly the functions of cellular respiration in the context of photosynthesis and symbiotic nitrogen fixation. Several of these functions depend on proteins and protein-protein interactions. We employ “complexome profiling” for systematically characterizing protein-protein interactions. This experimental approach is based on analyzing large numbers of protein fractions by proteomic shotgun-experiments, which requires a fast mass spectrometry system. Furthermore, we plan to investigate the functions of organelles of specific cell types of plant organs. For this, mitochondria are marked with tagged proteins in a cell-type specific manner, which allows their targeted isolation. Resulting protein fractions are of very low quantity and their proteomic analysis requires a highly sensitive mass spectrometry system. Finally, signal transduction pathways regulating molecular functions of plants shall be investigated, particularly those steps which are based on the binding of ligands to specific protein targets. Experiments are based on using „Tandem mass tags“ (TMTs) and require a high-resolution mass spectrometry system. The requested mass spectrometry system is perfectly suitable for the planned research projects. The research described here will give new insights into molecular functions of plants relevant for increasing plant yield and for improving plant stress tolerance.

汉诺威莱布尼茨大学自然科学学院植物蛋白质组学系的研究重点是蛋白质和蛋白质组水平上的植物分子过程。蛋白质质谱是我们项目的核心。此外，我们的部门还从事分析来自莱布尼茨大学汉诺威赫伦豪森校区其他各研究所的蛋白质组分。目前，我们部门正在运行两个质谱系统用于蛋白质分析，这是用来能力。在过去的十年中，质谱系统有了很大的发展。新的质谱分析系统将大大提高我们的研究能力，并允许深入研究植物的分子功能，这是我们目前无法做到的。植物蛋白质组学系特别感兴趣的是探索植物的能量生物学，特别是在光合作用和共生固氮的背景下细胞呼吸的功能。这些功能中的一些依赖于蛋白质和蛋白质-蛋白质相互作用。我们采用“复合体分析”系统地表征蛋白质-蛋白质相互作用。这种实验方法基于通过蛋白质组学鸟枪实验分析大量蛋白质组分，这需要快速质谱系统。此外，我们计划研究植物器官的特定细胞类型的细胞器的功能。为此，线粒体以细胞类型特异性方式用标记蛋白质标记，这允许它们的靶向分离。所得蛋白质级分的量非常低，并且它们的蛋白质组学分析需要高度灵敏的质谱系统。最后，应研究调节植物分子功能的信号转导途径，特别是那些基于配体与特定蛋白质靶标结合的步骤。实验基于使用“串联质量标签”（TMT），需要高分辨率质谱系统。所要求的质谱系统非常适合计划中的研究项目。本文所述的研究将为植物的分子功能提供新的见解，这些功能与提高植物产量和改善植物抗逆性有关。