Application of ion channel biosensor to single neural cell analysis

我们已经开发了将平面膜片钳技术应用于单细胞分析所需的基本技术。研制了孵育型平面膜片钳器件，实现了(1)全细胞模式下配体门控离子通道电流的测量；(2)实现了光敏离子通道对HEK293的转导和克隆；(3)形成了神经网络并实现了光敏离子通道的转导；(4)研制了高稳定的平面膜片钳电极；(5)实现了激光照射产生激活电位。所有必要的基本技术都成功地开发出来了。

We have developed the elementary technology necessary to apply the planer patch clamp technology to the single neural cell analysis. Incubation type planar patch clamp device was developed and (1) Ligand gated ion channel current measurement by whole cell mode was attained, (2) Transfection of photosensitive ion channel to HEK293 and cloning were attained, (3) Neural network was formed and photosensitive ion channel was transfected, (4) Highly stable electrode of planar patch clamp was developed, and (5) Generation of active potential by laser irradiation was attained. All necessary elementary technologies were successfully developed.