Application of ion channel biosensor to single neural cell analysis

我们已经开发了将刨床膜片钳技术应用于单个神经细胞分析所需的基本技术。研制了培养型平面膜片钳装置，实现了(1)全细胞模式配体门控离子通道电流测量；(2)光敏离子通道转染HEK293并克隆；(3)形成神经网络并转染光敏离子通道；(4)研制了高度稳定的平面膜片钳电极；(5)实现了激光照射产生活性电位。所有必要的基础技术都已成功开发。

We have developed the elementary technology necessary to apply the planer patch clamp technology to the single neural cell analysis. Incubation type planar patch clamp device was developed and (1) Ligand gated ion channel current measurement by whole cell mode was attained, (2) Transfection of photosensitive ion channel to HEK293 and cloning were attained, (3) Neural network was formed and photosensitive ion channel was transfected, (4) Highly stable electrode of planar patch clamp was developed, and (5) Generation of active potential by laser irradiation was attained. All necessary elementary technologies were successfully developed.