Development and Evaluation of Integron Gene Cassette Metagenome Analysis

该项目旨在首次在极端海洋环境中研究可移动的DNA元件、整合子和相关基因盒。新的和多样化的整合子结构和环境适应性基因盒已经恢复。这个项目有三个主要成就。首先，介绍了从环境宏基因组中恢复整合子的新方法。其次，它扩展了我们对整合子在细菌适应极端海洋条件中的作用的理解。第三，它为我们提供了一些新的适应性和代谢基因，这些基因具有多种生物技术应用。

This project aimed to study the mobile DNA element, integrons, and associated gene cassettes, for the first time, in extreme marine environments. Novel and diverse integron structures and environment- adaptive gene cassettes have been recovered. This project had three main achievements. First, it introduced new methodology for recovery of integrons from environmental metagenomes. Second, it extended our understanding to the contribution of integrons in the adaptation of bacteria to extreme marine conditions. Third, it provided us with several novel adaptive and metabolic genes that have several biotechnological applications.