Development of innovative method for diagnosing renal disease -Urinary Molecular Profiling-

开发诊断肾脏疾病的创新方法-尿液分子分析-

• 批准号: 21880002
• 负责人: ICHII Osamu
• 金额: $ 1.76万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21880002/
• 关键词: バイオマーカー; 尿; 慢性腎臓病; 伴侶動物; mRNA; microRNA; 糸球体傷害; 尿細管間質病変; Luminal Epithelial Deciduation (LED); 慢性腎臓病(CKD); PCRarray/microarray; 糸球体上皮細胞傷害; mRNA/microRNA; イヌ/ネコ; 管腔上皮脱落(LED); 疾患モデルマウス; Interleukin 1 family member 6; mRNA・microRNA; PCRarra

This study clarified that the renal parenchymal cells were dropped into urine in renal pathological conditions. The quantitative and qualitative urinary cellular patterns correlated with the deteriorations of renal pathology and function in renal disease models and companion animals. Importantly, these urinary cells could be detected by molecular biological methods targeting mRNA and microRNA in urine. These data indicated the possibility that the urinary tests based on the detections of urinary cells, entitled "Urinary Molecular Profiling" in the present study, could be noninvasive diagnosis methods for renal disease.

本研究阐明在肾脏病理条件下，肾实质细胞掉落到尿液中。肾脏疾病模型和伴侣动物的尿细胞定量和定性模式与肾脏病理和功能的恶化相关。重要的是，这些尿细胞可以通过靶向尿液中mRNA和microRNA的分子生物学方法来检测。这些数据表明，基于尿细胞检测的尿液检测，在本研究中称为“尿液分子谱”，可能是肾脏疾病的无创诊断方法。

项目成果

129+Ter/Svマウスの遺伝的背景は腎糸球体硬化症に抵抗性である

129+Ter/Sv小鼠的遗传背景对肾小球硬化具有抵抗力。

• 发表时间: 2011
• 作者: 西野智博、市居修;他
• 通讯作者: 他

腎疾患の新しい診断指標-Interleukin 1 family, member 6 は尿細管傷害マーカーになり得る

肾脏疾病新诊断指标——白细胞介素1家族成员6可作为肾小管损伤的标志物

• 发表时间: 2009
• 作者: 市居修;他
• 通讯作者: 他

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Quantitative and Qualitative Urinary Cellular Patterns Correlate with Progression of Murine Glomerulonephritis

• DOI: 10.1371/journal.pone.0016472
• 发表时间: 2011-01-31
• 期刊: PLOS ONE
• 影响因子: 3.7
• 作者: Kimura, Junpei;Ichii, Osamu;Kon, Yasuhiro
• 通讯作者: Kon, Yasuhiro

Overexpression of interferon-activated gene 202 (Ifi202) correlates with the progression of autoimmune glomerulonephritis associated with the MRL chromosome 1

• DOI: 10.1177/0961203310362534
• 发表时间: 2010-07-01
• 期刊: LUPUS
• 影响因子: 2.6
• 作者: Ichii, O.;Kamikawa, A.;Kon, Y.
• 通讯作者: Kon, Y.