Enhancement of gene targeting via cell cycle regulation in higher plants

点击翻译按钮获取中文摘要

Gene targeting is achieved through the homologous recombination between endogenous genome and foreign DNA, which has homology to endogenous genome. Homologous recombination is one of the DNA double strand break repair mechanism. In contrast to prokaryotes and lower eukaryotes, gene targeting is infrequent in higher plants and foreign DNA integrates into plant genome mainly via non-homologous end joining because of low homologous recombination frequency. Depend on the cell cycle phase, the choice of one of the DNA repair pathway over the other will secure genome stability maintenance and homologous recombination is an efficient way to maintain genome stability at a post-replication stage. In this study, we analyzed the Arabidopsis mutants of cell cycle regulation factors and revealed that the mutant, which lacks positive regulator of G1/S transition showed low homologous recombination frequency.