Innovation of the novel cardiac regeneration therapy using gene introduction-mediated cell conversion into cardiac progenitor cells.

利用基因导入介导的细胞转化为心脏祖细胞的新型心脏再生疗法的创新。

• 批准号: 21890117
• 负责人: KAWAMURA Teruhisa
• 金额: $ 1.66万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21890117/
• 关键词: 分子心臓病態学; 再生医学; 幹細胞生物学; 体細胞初期化

Recent technologies to reprogram somatic cells into induced pluripotent stem (iPS) cells have been expected as a powerful tool of regeneration therapy for end-staged heart failure. However, duration of iPS cell derivation and the risk of tumorigenesis including teratoma after cell implantation as well as limited efficiency for cardiac differentiation are considered as great barriers against the clinical application of this powerful approach. To overcome these problems, by modifying the reprograming process, here we show the novel alternative strategy which enables us to create cardiac myocytes directly from fibroblasts rapidly and efficiently with a low risk of tumorigenesis. In addition, we established the mouse experimental model to evaluate the effect of this strategy as an innovative cardiac regeneration therapy.

体细胞重编程成诱导多能干细胞（iPS）的最新技术有望成为终末期心力衰竭再生治疗的有力工具。然而，iPS细胞分化的持续时间、细胞植入后发生畸胎瘤等肿瘤的风险以及有限的心脏分化效率被认为是这种强大方法临床应用的巨大障碍。为了克服这些问题，通过修改重编程过程，我们展示了一种新的替代策略，使我们能够直接从成纤维细胞快速有效地产生心肌细胞，并具有低肿瘤发生风险。此外，我们建立了小鼠实验模型来评估该策略作为一种创新的心脏再生疗法的效果。

项目成果

Cyclin-Dependent Kinase 9 Forms a Complex With GATA4 and Is Involved in the Differentiation of Mouse ES Cells Into Cardiomyocytes

• DOI: 10.1002/jcp.22336
• 发表时间: 2011-01-01
• 期刊: JOURNAL OF CELLULAR PHYSIOLOGY
• 影响因子: 5.6
• 作者: Kaichi, Shinji;Takaya, Tomohide;Hasegawa, Koji
• 通讯作者: Hasegawa, Koji

MicroRNA-27a regulates beta cardiac myosin heavy chain gene expression by targeting thyroid hormone receptor betal in neonatal rat ventricular myocytes.

MicroRNA-27a 通过靶向新生大鼠心室肌细胞中的甲状腺激素受体 β 来调节 β 心肌肌球蛋白重链基因表达。

• 发表时间: 2011
• 期刊: Mol Cell Biol. 31
• 作者: Nishi H;Ono K;Horie T;Nagao K;Kinoshita M;Kuwabara Y;Watanabe S;Takaya T;Tamaki Y;Takanabe-Mori R;Wada H;Hasegawa K;Iwanaga Y;Kawamura T;Kita T;Kimura T.
• 通讯作者: Kimura T.

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