Examination of novel epitopes and development of inhibitory antibodies for the effective inhibition of HIV infection

为了检测可以有效抑制HIV感染的新表位，我们将HIV感染过程中在CD4T细胞细胞膜上形成的HIV包膜-CD4-趋化因子受体的膜融合中间产物免疫给产生高亲和力抗体(Ab)的小鼠。并建立了抗CD4单抗和抗融合复合体(Fc)单抗文库。抗CD4R275单抗可识别新的CD43区表位，并能抑制HIV-1感染。我们建立了识别膜融合过程中动态表位的抗Fc单抗文库。我们的mAb文库可用于检测抑制HIV感染的新靶点。

For the examination of novel epitopes that can effectively inhibit HIV infection, we immunized soluble CD4 and membrane fusion intermediate complex of HIV envelope-CD4-chemikine receptor that is formed on the cell membrane of CD4+ T cell during the infection of HIV, to the high-affinity antibody (Ab) producing mice. And we established anti-CD4 monoclonal (m) Ab and anti-fusion complex (FC) mAb libraries. Anti-CD4 R275 mAb recognized novel epitope of domain three of CD4 and inhibited HIV-1 infection. We established anti-FC mAb library that recognized dynamic epitopes appearing during the membrane fusion process. Our mAb library may be useful on the examination of novel target of inhibition of HIV infection.