Elucidation of the periodontal disease using cell cycle-arrested quiescent osteoclast precursors(QOP).

在破骨细胞的分化过程中，我们分析了破骨细胞前体细胞（cell cycle-arrested spleased osteoclast precursors，QOP）的特性，即在没有细胞周期的情况下向破骨细胞分化。本研究通过骨形态发生蛋白-2（BMP）分析异位成骨时QOP的存在方式，检测QOP是否存在于血液和骨髓中。QOP巨噬细胞标志物表达低，破骨细胞标志物表达高。电镜下观察到与破骨细胞相似的图像。此外，QOP出现在异位骨周围。因此，QOP有可能是破骨细胞的特异性前体。在现有的细胞周期中停止的QOP特异性地将形成的破骨细胞迁移到破骨细胞形成部分的异位骨，并且认为I通过骨吸收刺激分化为破骨细胞。这一概念与牙周病等炎性骨破坏病变的骨吸收因子减少的概念不同，认为有助于阐明新的骨病机制。

In a differentiation process of the osteoclast, We analyzed the character of osteoclast precursor cell (cell cycle-arrested quiescent osteoclast precursors : QOP) which differentiated to osteoclast without cell cycle. In this study, We analyzed an existence style of QOP at the time of the ectopic bone by bone morphogenetic protein-2(BMP) to examine whether QOP existed in blood as well as bone marrow. QOP had a low expression of macrophage marker, but had a high osteoclast marker. In addition, QOP got the views that were near to osteoclast by an electron microscope. Furthermore, QOP appeared around an ectopic bone. Therefore, QOP have a possibility that it is a specific precursor for osteoclast. And QOP which stopped in an existing cell cycle specifically migrates the osteoclast formed to an ectopic bone in the osteoclast formation part, and it is thought that I differentiate to osteoclast by bone resorption stimulation. This concept prints a different concept that decrease of bone resorption factor for inflammatory bone destruction lesions such as periodontal disease and thinks that I make a help to elucidation of new bone disease mechanism.