Elucidation of constructing and working mechanisms of histone modification network

组蛋白修饰网络的构建和工作机制的阐明

• 批准号: 22870007
• 负责人: HAYASHI Yohei
• 金额: $ 1.05万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22870007/
• 关键词: エピジェネティクス; 複雑ネットワーク; 蛋白質; 化学修飾; 頑強性; ヒストン

Our previous theory on histone modifications suggested that mutual controls among a wide variety of histone modifications form complex histone modification network. In this study, to elucidate how the network is built and working in iving cells, the aberrances of the network caused by the point mutation of histone modifiable residues were comprehensively examined as phenotypic changes using the histone point-mutant library of the budding yeast. As a result, histone H3-Lys4, -Lys36 and histone H4-Lys12, -Lys16 were identified as modifiable residues working as "functional hubs", which play an important role in the maintenance of biological functions.

我们以前的组蛋白修饰理论认为，多种组蛋白修饰之间的相互控制形成了复杂的组蛋白修饰网络。本研究利用芽殖酵母的组蛋白点突变文库，将组蛋白可修饰残基的点突变引起的网络异常作为表型变化进行了全面的研究，以阐明该网络是如何在活细胞中构建和工作的。因此，组蛋白H3-Lys 4，-Lys 36和组蛋白H4-Lys 12，-Lys 16被鉴定为作为“功能枢纽”的可修饰残基，其在维持生物学功能中起重要作用。

项目成果

Network-based histone "modification web" theory and "DESS" strategy for elucidating the physiological significance of histone modifications.

基于网络的组蛋白“修饰网”理论和“DESS”策略阐明了组蛋白修饰的生理意义。

• 发表时间: 2011
• 作者: Hayashi Y;Horikoshi M
• 通讯作者: Horikoshi M

Mechanistic models on eukaryotic gene regulation.

真核基因调控的机制模型。

• 发表时间: 2010
• 作者: Hayashi Y;Sato L;Kawashima S;Akai Y;Adachi N;Natsume R;Senda T;Seki M;Horikoshi M
• 通讯作者: Horikoshi M

Global analysis for functional residues of histone variant Htz1 using the comprehensive point mutant library

使用综合点突变文库对组蛋白变体 Htz1 的功能残基进行全局分析

• DOI: 10.1111/j.1365-2443.2011.01512.x
• 发表时间: 2011
• 期刊: Genes Cells
• 影响因子: 2.1
• 作者: A.Kawano;Y.Hayashi;S.Noguchi;H.Handa;M.Horikoshi & Y.Yamaguchi
• 通讯作者: M.Horikoshi & Y.Yamaguchi

Global analysis of functional relationships between histone point mutations and the effects of histone deacetylase inhibitors

• DOI: 10.1111/j.1365-2443.2010.01408.x
• 发表时间: 2010-06
• 期刊: Genes to Cells
• 影响因子: 2.1
• 作者: L. Sato;Shuhei Noguchi;Y. Hayashi;M. Sakamoto;M. Horikoshi

Global analysis for functional residues of histone variant Htzl using the comprehensive point mutant library.

使用综合点突变文库对组蛋白变体 Htzl 的功能残基进行全局分析。

• 发表时间: 2011
• 期刊: Genes Cells
• 影响因子: 2.1
• 作者: A.Kawano;et al.
• 通讯作者: et al.