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Examining the mechanisms underlying the abiotic stress tolerance of the wild tomato species Solanum pennellii

Examining the mechanisms underlying the abiotic stress tolerance of the wild tomato species Solanum pennellii
研究野生番茄品种 Solanum pennellii 的非生物胁迫耐受性机制
批准号:
452682775
负责人:
Professor Dr. Alisdair Fernie, Ph.D.
金额:
$0.0万
依托单位国家:
德国
项目类别:
Research Grants
财政年份:
--
资助国家:
德国
项目状态:
未结题
起止时间:

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中文摘要
翻译
野生番茄具有显著的非生物抗逆性。至少在龙葵中,这可能是对其相对恶劣的自然栖息地的一种适应。有趣的是,龙葵的耐逆性似乎与转座元件的插入呈正相关。也有证据表明,不仅在西红柿中存在代谢核心压力反应,这可能会导致压力反应的改善。在这个项目中,我们将首先对“番茄遗传资源中心”收集的近50个不同的龙葵品系进行盐和干旱胁迫反应的改良研究,并产生这些品系的PAN(Epi)基因组。然后,将使用系统生物学方法对选定的品系进行详细分析,以找出胁迫反应的分子关系,特别是转座子的作用。在一种互补的方法中,含有彭尼利链霉菌基因组一小部分的选定栽培番茄品系将被用来寻找介导逆境反应的基因/转座子。最后,将对识别出的基因进行第一次验证实验。
英文摘要
Wild tomato species are characterized by a remarkable abiotic stress tolerance. At least in Solanum pennellii this is likely an adaptation to its natural relatively harsh habitat. Interestingly, there seems to be a positive correlation between the stress tolerance of Solanum pennellii and the insertion of transposable elements. There is also evidence for a metabolic core stress response not only in tomatoes, which may lead to an improved stress response. In this project, almost 50 different Solanum pennellii lines in the collection of the "Tomato Genetic Resource Center" will first be investigated for an improved salt and drought stress response and a pan(epi)genome of these lines will be generated. Selected lines will then be analyzed in detail using a systems biology approach to find the molecular relationship of the stress response and especially the role of the transposons. In a complementary approach, selected cultivated tomato lines harboring a small part of the S. pennellii genome will be used to find genes/transposons that mediate stress response. Finally, first validation experiments with the identified genes will be performed.
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