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Examining the mechanisms underlying the abiotic stress tolerance of the wild tomato species Solanum pennellii

Examining the mechanisms underlying the abiotic stress tolerance of the wild tomato species Solanum pennellii
研究野生番茄品种 Solanum pennellii 的非生物胁迫耐受性机制
批准号:
452682775
负责人:
Professor Dr. Alisdair Fernie, Ph.D.
金额:
$0.0万
依托单位国家:
德国
项目类别:
Research Grants
财政年份:
--
资助国家:
德国
项目状态:
未结题
起止时间:

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中文摘要
翻译
野生番茄品种具有显著的非生物抗逆性。至少在pennellii中,这可能是对其相对恶劣的自然栖息地的适应。有趣的是,pennellium的抗逆性似乎与转座因子的插入有正相关。也有证据表明,代谢核心应激反应不仅存在于西红柿中,这可能导致应激反应的改善。在本项目中,将首先对“番茄遗传资源中心”收集的近50个不同的pennellii品系进行改进盐胁迫和干旱胁迫响应的研究,并生成这些品系的pan(epi)基因组。然后将使用系统生物学方法对选定的细胞系进行详细分析,以发现应激反应的分子关系,特别是转座子的作用。在一种互补的方法中,选择含有一小部分pennellii基因组的栽培番茄品系将用于寻找介导应激反应的基因/转座子。最后,将对鉴定的基因进行首次验证实验。
英文摘要
Wild tomato species are characterized by a remarkable abiotic stress tolerance. At least in Solanum pennellii this is likely an adaptation to its natural relatively harsh habitat. Interestingly, there seems to be a positive correlation between the stress tolerance of Solanum pennellii and the insertion of transposable elements. There is also evidence for a metabolic core stress response not only in tomatoes, which may lead to an improved stress response. In this project, almost 50 different Solanum pennellii lines in the collection of the "Tomato Genetic Resource Center" will first be investigated for an improved salt and drought stress response and a pan(epi)genome of these lines will be generated. Selected lines will then be analyzed in detail using a systems biology approach to find the molecular relationship of the stress response and especially the role of the transposons. In a complementary approach, selected cultivated tomato lines harboring a small part of the S. pennellii genome will be used to find genes/transposons that mediate stress response. Finally, first validation experiments with the identified genes will be performed.
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