Study onthe influenza virusmatrix protein incorporation to virion.

我们在HEK 293T细胞中同时表达Flag标记的M1（M1- fl）和HA标记的M1(HA-M1)，并使用抗Flag抗体进行免疫沉淀。在沉淀物中，用抗ha抗体进行westernblot检测HA-M1。在该体系中对A/ Udorn/ 72（H3N2）丝状病毒的M1进行分析，发现IP缓冲液的pH值不影响络合物的形成。另一方面，来自A/ WSN/ 33（H1N1）的M1形成球形颗粒，形成对环境pH敏感的复合物。

We expressed Flag tagged M1(M1-FL) and HA tagged M1(HA-M1) together in HEK 293T cells and immunoprecipitated using anti-Flag antibody. In the precipitate, HA-M1 was detected in the westernblot analysis with anti-HA antibody. When M1 from filamentous virus A/ Udorn/ 72(H3N2) was analyzed in this system, it was shown that pH of the IP buffer did not affect the formation of complex. On the other hand, M1 from A/ WSN/ 33(H1N1), which forms spherical particle, formed the complex which showed susceptibility to the environmental pH.