Functional analysis of cancer-specific human T cells in NOG mice : Cell interaction of CD4, CD8 cells

用于癌症的免疫细胞疗法（ACT）越来越成为临床试验的主题，其中T细胞从患者的外周血收集，通过肿瘤抗原特异性T细胞受体（TCR）的转导进行修饰，并重新施用给同一患者。在本研究中，我们的目的是建立一个小鼠模型来分析“体内”功能的转移T细胞通过利用免疫功能低下（NOG）小鼠。我们观察到移植的人食管肿瘤（HLA-A2402+，MAGE-A4+）生长的消退仅当共施用经修饰以表达TCR（在HLA-A2402的背景下识别MAGE-A4（p143-151）肽）的T细胞时。这一模型将为我们提供关于转移T细胞的功效机制的更详细的信息。

Adoptive cell therapy(ACT) for cancer, in which T cells are collected from patient's peripheral blood, modified by the transduction of tumor-antigen specific T cell receptor(TCR) and re-administered to the same patient, is increasingly the subject of clinical trials. In this study, we aimed to establish a mouse model to analyze "in vivo" functions of the transferred T cells by utilizing immunocompromised(NOG) mice. We observed the regression of transplanted human esophageal tumor(HLA-A2402+, MAGE-A4+) growth only when T cells modified to express TCR, recognizing MAGE-A4(p143-151) peptide in the context of HLA-A2402, were co-administered. This model will provide us more detailed information about mechanism of efficacies of transferred T cells.