Triple-Quadrupol Mass spectrometer

化合物的定性和定量分析在药物研究中具有重要意义。波恩大学以研究为导向的药学院通过创新药物和药物疗法研究中心波恩医药中心广泛联系在一起，并在生命和健康科学领域发挥核心作用。因此，我们要求一台用于药学院化学分析平台的三重四极质谱计，用于灵敏的小分子定量分析。由于目前可用的仪器不足，几个主要由第三方资助的联合项目(如GRK1873、FOR2372、FOR2625、FOR2685、SFB1328、TR-SFB259、TR-SFB261)迫切需要这种MS仪器。要处理的分析问题范围很广，从有机物质和动物组织中药物浓度和代谢物的定量测定，到化石中有机化合物的分析，再到创新的蛋白质组学方法。化学分析平台隶属于药物和药物化学系，为整个药学院、波恩医药中心和其他地方提供分析服务。定量分析所需的质谱计是对邻近的、密切合作的化学研究所现有的质谱仪的补充，这些仪器侧重于定性分析和成像。新的质谱仪将在各学系之间和(国际)国家项目的框架内跨学科地使用。除药剂部外，还直接参与了本申请。&Med.医学系设有分子生物学和生物化学研究所(吉塞尔曼，温特)和药理与毒理研究所(费费弗)。设想的用户组包括高级、经验丰富的科学家以及年轻的有才华的科学家(CrüSemann，Engeser)，将确保仪器完全用于复杂项目的工作。由于药学专业的学生被培养成药物分析专家，包括仪器分析，MS仪器也计划用于教学(本科生研究项目和后续的硕士论文)。选择最佳体系的重要标准包括(I)高灵敏度和线性，(Ii)灵活性，(Iii)质量可达2000D，以及(Iv)异构体的测定。

The qualitative and quantitative analysis of chemical compounds is of central importance in drug research. The research-oriented Faculty of Pharmacy at the University of Bonn is broadly interconnected via the PharmaCenter Bonn, a Research Center for Innovative Drugs and Pharmacotherapy, and plays a central role in the area of life and health sciences. Hereby, we request a triple-quadrupol mass spectrometer for the platform „Chemical Analytics“ of the Faculty of Pharmacy for the sensitive quantitative analysis of small molecules. This MS instrument is urgently required for several, mostly third party-funded joint projects (e.g. GRK1873, FOR2372, FOR2625, FOR2685, SFB1328, TR-SFB259, TR-SFB261), since the currently available instruments are insufficient. A broad spectrum of analytical problems is to be handled, ranging from the quantitative determination of drug concentrations and metabolites in organoids and animal tissues, and the analysis of organic compounds in fossils, to innovative proteomics approaches. The platform for Chemical Analytics, which is associated with the Department of Pharmaceutical & Medicinal Chemistry, offers analytical service for the entire Faculty of Pharmacy, the PharmaCenter Bonn and beyond. The required mass spectrometer for quantitative analysis is complementary to the MS instruments that are available at the neighbouring, closely collaborating Chemical Institutes, which are focused on qualitative analysis and imaging.The new mass spectrometer will be utilized interdisciplinarily, between faculties and in the frame of (inter)national projects. Directly involved in the present application are besides the Department of Pharm. & Med. Chemistry (Müller, Gütschow), the Department of Pharmaceutical and Cell-Biological Chemistry (Hansen, Bendas), the Institute of Pharmaceutical Biology (König, Crüsemann), the Institute of Organic Chemistry (Menche, Engeser), and the Medical Faculty with the Institute of Molecular Biology and Biochemistry (Gieselmann, Winter) and the Institute of Pharmacology & Toxicology (Pfeifer). The envisaged user group, comprising high-ranking, experienced scientists as well as young talented scientists (Crüsemann, Engeser), will ensure complete occupancy of the instrument for work on sophisticated projects. Since students of Pharmacy are trained to become experts for Pharmaceutical Analytics including Instrumental Analytics, the MS instrument is also planned to be used for teaching (undergraduate research projects, and subsequent Master theses). Important criteria for the selection of the optimal system include (i) high sensitivity and linearity, (ii) flexibility, (iii) masses up to 2000 D, and (iv) determination of isomers.