5-Laser Flow cytometer

5-激光流式细胞仪

• 批准号: 514808265
• 金额: --
• 依托单位: Universität Ulm
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/514808265?language=en
• 关键词: Laser; Flow; cytometer

In the field of biomedical research, multi-parameter flow cytometry represents an indispensable technology for the clarification of fundamental immunobiological processes as a basis to develop new therapeutic approaches. Immunological aspects are coming to the fore in the most diverse directions of research, such as in the fields of neurology, aging and trauma research as well as in oncological basic and translational research - focal points of the research location Ulm. Being able to carry out cutting-edge research requires not only outstanding ideas and concepts, but also highly specialized equipment. The high-performance flow cytometer applied here (5 lasers, 30 fluorescence detectors, 2 scattered light detectors) is required to comprehensively - phenotypically and functionally - characterize rare and complex cell populations. Furthermore, the limitation in the sample material requires a simultaneous analysis of a large number of parameters. The need for a comprehensive characterization of heterogeneous immune cell populations also arises from the parallel investigation of the transcriptome at the single cell and population level. The projects of the working groups involved in this application absolutely require the existence of a high-performance flow cytometer as applied here in order to be able to realize the basic-oriented and translational research projects and to be able to further develop internationally and competitively.The high demand for a high-performance flow cytometer and the high acquisition costs speak in favor of integrating this device into the existing central core facility "Immune Monitoring", which offers all interested scientists optimal full service in the field of immune monitoring of humans and mice across all institutions. The integration of this device in the central core facility "Immune Monitoring" enables efficient use of the device as well as expert support for the user from planning to implementation and evaluation of the generated data. At present, the core facility "Immune Monitoring", equipped with a 3-laser device from Beckman Coulter (Gallios Flow Cytometer; 10 colors), has reached the limits of processing current and creative questions. The use of a more powerful analytical flow cytometer, equipped with 5 lasers and the possibility of detecting up to 30 colors, significantly increases the quality of the research results. Moreover, this opens up opportunities to implement complex, contemporary and innovative research approaches. This enables us to achieve therapeutically significant scientific results and on the other hand, to be able to persist in the competition for the acquisition of national and international joint and individual funding measures.

在生物医学研究领域，多参数流式细胞术代表了一种不可或缺的技术，用于澄清基本的免疫生物学过程，作为开发新治疗方法的基础。免疫学方面正在最多样化的研究方向中脱颖而出，例如在神经病学，衰老和创伤研究领域以及肿瘤学基础和转化研究领域-研究地点乌尔姆的焦点。能够开展前沿研究不仅需要杰出的思想和理念，还需要高度专业化的设备。这里应用的高性能流式细胞仪（5个激光器、30个荧光检测器、2个散射光检测器）需要全面表征稀有和复杂的细胞群的表型和功能。此外，样品材料的限制要求同时分析大量参数。对异质性免疫细胞群体的全面表征的需要也来自于在单细胞和群体水平上对转录组的平行研究。参与该应用的工作组的项目绝对需要存在如本文所应用的高性能流式细胞仪，以便能够实现基础导向和转化研究项目，并且能够进一步在国际上发展并具有竞争力。高性能流式细胞仪和高购置成本支持将该设备集成到现有的中央核心设施“免疫监测”中，它为所有感兴趣的科学家提供了所有机构在人类和小鼠免疫监测领域的最佳全面服务。将该设备集成到中央核心设施“免疫监测”中，可以有效使用该设备，并为用户提供从规划到实施和评估生成数据的专家支持。目前，核心设施“免疫监测”配备了贝克曼库尔特的3激光设备（Gallios流式细胞仪; 10种颜色），已达到处理当前和创造性问题的极限。使用更强大的分析流式细胞仪，配备5个激光器和检测多达30种颜色的可能性，显着提高了研究结果的质量。 此外，这为实施复杂，现代和创新的研究方法提供了机会。这使我们能够取得具有治疗意义的科学成果，另一方面，能够坚持争取国家和国际联合和单独供资措施。