5-laser Flow Cytometer

在这份提案中，我们正在为慕尼黑Klinikum rechts der Isar内科II的研究小组申请一台5激光流式细胞仪。这种流式细胞仪将极大地增强参与研究小组的实验可能性。流式细胞术是一种能够对活细胞群体进行快速、高效和差异化分析的技术，是免疫学研究的核心方法。到目前为止，该部门唯一可用的流式细胞仪已有10多年的历史，可以同时分析10个通道。然而，这里提出的设备提供了同时分析多达40个不同的荧光团的可能性。免疫细胞群体日益多样化，往往需要大量不同的标记物来描述其功能和表型。与此同时，分析通常是在有限数量的主要材料中进行的，特别是在处理患者样本时。因此，高性能的流式细胞仪对于识别复杂的免疫相互作用是必不可少的，特别是在患者材料中。参与这项提议的研究小组负责人在流式细胞术方面拥有长期的专业知识，因此可以保证对提议的装置进行适当的处理。

In this proposal, we are applying for a 5 laser flow cytometer for the research groups of the Department of Internal Medicine II at Klinikum rechts der Isar, Munich. This flow cytometer will critically enhance the experimental possibilities of the participating research groups. Flow cytometry is a technique, which allows for a rapid, efficient and differentiated analysis of living cell populations and it is a central method in immunological research. To date, the only available flow cytometer at the department is more than 10 years old and allows for the simultaneous analysis of 10 channels. The device proposed here, however, offers the possibility of concurrently analyzing up to 40 different fluorophores. Immune cell populations are increasingly diverse, and a high number of different markers is often required for their functional and phenotypic characterization. At the same time, analyses are often conducted in a restricted amount of primary material, especially when working with patient samples. Therefore, a high-performance flow cytometer is indispensable for the identification of complex immunological interactions, especially in patient material. The research group leaders involved in this proposal have long-standing expertise in flow cytometry and can therefore reassure a proper handling of the proposed device.