5-laser Flow Cytometer

5激光流式细胞仪

• 批准号: 507583661
• 金额: --
• 依托单位: Technische Universität München (TUM)
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/507583661?language=en
• 关键词: laser; Flow; Cytometer

In this proposal, we are applying for a 5 laser flow cytometer for the research groups of the Department of Internal Medicine II at Klinikum rechts der Isar, Munich. This flow cytometer will critically enhance the experimental possibilities of the participating research groups. Flow cytometry is a technique, which allows for a rapid, efficient and differentiated analysis of living cell populations and it is a central method in immunological research. To date, the only available flow cytometer at the department is more than 10 years old and allows for the simultaneous analysis of 10 channels. The device proposed here, however, offers the possibility of concurrently analyzing up to 40 different fluorophores. Immune cell populations are increasingly diverse, and a high number of different markers is often required for their functional and phenotypic characterization. At the same time, analyses are often conducted in a restricted amount of primary material, especially when working with patient samples. Therefore, a high-performance flow cytometer is indispensable for the identification of complex immunological interactions, especially in patient material. The research group leaders involved in this proposal have long-standing expertise in flow cytometry and can therefore reassure a proper handling of the proposed device.

在本提案中，我们正在为慕尼黑Klinikum rechts der Isar的第二内科的研究小组申请一台5激光流式细胞仪。这种流式细胞仪将极大地提高参与研究小组的实验可能性。流式细胞术是一种快速、高效、差异化分析活细胞群的技术，是免疫学研究的核心方法。到目前为止，该部门唯一可用的流式细胞仪已经使用了10多年，可以同时分析10个通道。 然而，这里提出的设备提供了同时分析多达40种不同荧光团的可能性。免疫细胞群体日益多样化，并且其功能和表型表征通常需要大量不同的标记物。同时，分析通常是在有限数量的原始材料中进行的，特别是在处理患者样本时。因此，高性能流式细胞仪对于鉴定复杂的免疫相互作用是必不可少的，特别是在患者材料中。参与该提案的研究小组负责人在流式细胞术方面拥有长期的专业知识，因此可以确保正确处理所提议的设备。