Clarification of the mechanism for acceleration of new bone formation by an electrically polarized hydroxyapatite from the molecular biological point of view

从分子生物学角度阐明电极化羟基磷灰石加速新骨形成的机制

• 批准号: 25893236
• 负责人: OHBA Seiko
• 金额: $ 0.92万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2013
• 资助国家: 日本
• 起止时间: 2013-08-30 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-25893236/
• 关键词: 分極; ハイドロキシアパタイト; 骨芽細胞; 破骨細胞

By clarifying the osteoblastic cell behavior on polarized HA from the molecular biological point of view,we analized the effect on the bone of polarized HA and identification of gene concerning bone formation of polarized were done by microarray.As a result of microarray, there was a difference between same sample, we analyzed the differences between samples in n=1 in Excel and complied the data. Our future challenges are to use software for microarray, pick up and remove the genes that show big difference between n=2 in the same sample, and analyze the expression level between samples again to narrow down the high expression genes. We'd like to narrow down high expression genes by microarray comprehensively, analyze them by realtime PCR and westernblot, and clarify the genes relate to bone formation on the surface of electrically-polarized HA.

从分子生物学的角度阐明成骨细胞在极化HA上的行为，分析极化HA对骨的影响，并利用基因芯片技术对极化HA骨形成相关基因进行鉴定，结果发现同一样本间存在差异，在Excel中对n=1的样本间差异进行分析，整理数据。我们未来的挑战是使用微阵列软件，在同一样本中挑选并去除n=2差异较大的基因，并再次分析样本之间的表达水平，以缩小高表达基因的范围。本研究拟通过基因芯片技术全面筛选高表达基因，并通过实时荧光PCR和Westernblot技术进行分析，明确与电极化HA表面成骨相关的基因。