Clarification of the mechanism for acceleration of new bone formation by an electrically polarized hydroxyapatite from the molecular biological point of view

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By clarifying the osteoblastic cell behavior on polarized HA from the molecular biological point of view,we analized the effect on the bone of polarized HA and identification of gene concerning bone formation of polarized were done by microarray.As a result of microarray, there was a difference between same sample, we analyzed the differences between samples in n=1 in Excel and complied the data. Our future challenges are to use software for microarray, pick up and remove the genes that show big difference between n=2 in the same sample, and analyze the expression level between samples again to narrow down the high expression genes. We'd like to narrow down high expression genes by microarray comprehensively, analyze them by realtime PCR and westernblot, and clarify the genes relate to bone formation on the surface of electrically-polarized HA.