Joint Research on Microbial Enhancement Oil Recovery.

微生物强化采油联合研究。

• 批准号: 10044116
• 负责人: CHIDA Tadashi
• 金额: $ 4.99万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10044116/
• 关键词: MEOR; Oil; Enhanced Recovery; Jilin oilfield; Field trial; Natural Microorganisms; Nutritive salts; Direct-PCR; 増進回収法; ポリマー生産; PCR-RFLP

Tohoku University and Jilin Petroleum Group Co., China have been cooperatively proceeded a research project on microbial enhanced oil recovery (MEOR) processes in order to produce additional crude oil from oil reservoirs underground. The objective of this study was to evaluate the feasibility of the MEOR process through the experiment using both laboratory and actual oil reservoir. Field trials with flooding methods were carried out in a waterflooded reservoir of Fuyu-oilfield, China. Strain CJF-002 was injected to the reservoir with nutritive salts (molasses and inorganic compounds). The strain was isolated from reservoir rock of Fuyu-oilfield, identified as Enterobacter sp., and has an ability of producing insoluble polymer, which is expected to improve of sweep efficiency caused by plugging high permeability zone. MPN-Direct-PCR method and fluorescence in situ hybridization (FISH) method were used for rapid detection of the bacterium in the field trials. Field trials with huff-and-puff methods were also carried out with injecting nutritive salts only or with injecting nutritive salts and the bacterium. In some trials without injecting the strain CJF-002, natural microorganisms in the reservoir were activated and the oil production rate remarkably inceased due to the increase of the rate of fluid production.

东北大学和吉林石油集团公司，为了从地下油藏中开采出更多的原油，我国与国外合作开展了微生物采油技术的研究项目。本研究的目的是通过实验室和实际油藏的实验来评价微生物采油工艺的可行性。在扶余油田某水驱油藏进行了不同驱油方法的矿场试验。将菌株CJF-002与营养盐（糖蜜和无机化合物）一起注入储层。从扶余油田储层岩石中分离到一株细菌，经鉴定为肠杆菌属（Enterobacter sp.），并且具有产生不溶性聚合物的能力，这有望提高由堵塞高渗透层引起的波及效率。采用MPN直接PCR法和荧光原位杂交法对该菌进行了田间快速检测。还进行了仅注射营养盐或注射营养盐和细菌的吹气法田间试验。在不注入菌株CJF-002的一些试验中，由于产液速率的增加，储层中的天然微生物被激活，并且产油速率显著增加。

项目成果

Hideharu YONEBAYASHI et al.: "Screening of Microorganisms for Microbial Oil Recovery Processes"石油学会誌. 43・1. 59-69 (2000)

Hideharu YONEBAYASHI 等人：“微生物石油采收过程的微生物筛选”，日本石油学会杂志 43・1（2000 年）。

千田佶: "微生物を利用した新しい石油採取法"バイオサイエンスとバイオインダストリー. 58・6. 21-24 (2000)

Kei Chida：“使用微生物的新油提取方法”生物科学和生物工业58・6（2000）。

Yuichi Sugai: "Fundamental Studies on Microbial Oil Recovery with Polymer Producer Clostridium sp.TU-15A."Proceedings of International Symposium on Microbial Enhanced Oil Recovery. ISRE2000. 39-45 (2000)

Yuichi Sugai：“利用聚合物生产梭状芽胞杆菌 TU-15A 进行微生物采油的基础研究”。微生物采油国际研讨会论文集。

洪承燮: "油層の先住微生物を利用したMEOR研究"石油技術協会誌. 63・5. 432-441 (1998)

Hong Seung-seop：“利用油藏中的本土微生物进行 MEOR 研究”石油技术协会杂志 63・5（1998 年）。

菅野明 et al.: "MEORフィールドテストを想定したTU-7A株の代謝活性の推定"石油技術協会誌. 64・3. 233-241 (1999)

Akira Kanno 等：“假定 MEOR 现场试验的 TU-7A 菌株的代谢活性的估计”日本石油技术协会杂志 64・3（1999 年）。