Establishment of an analytical method for bacterial behaviors in an oil reservoir.

本工作的目的是建立油藏中细菌行为的分析方法。采用扫描化学显微镜（SCHEM，pH-成像显微镜）的方法，在假油库制成的填充床细胞中进行细菌行为的测量和评估。在填充有粒径为100-1,000μm硅砂的测量细胞中，可以使用扫描化学显微镜定量测定乳酸的传质。接下来，在同一细胞中接种干酪乳杆菌，通过显微镜测量细胞内的pH值。结果是，当细胞充满一些较小尺寸的颗粒时，pH 值变化更大。该现象是在使用较小尺寸的颗粒（45-900μm）用于填充床电池的情况下观察到的。另一方面，在使用某些尺寸颗粒的填充床柱中，细菌的生长量更大并且颗粒尺寸更小。在较小尺寸颗粒的情况下，从液体培养中细菌生长的产率因子计算，与较大尺寸相比，细菌密度增加至3-5倍。建立了表达二维填充床细胞中细菌生长和运输行为的数学模型，并通过该模型可以对这些现象进行充分的分析。因此，通过本研究的方法，确定了填充床细胞中细菌的生长行为。

It is Purpose of this work that establishment of an analytical method for bacterial behaviors in an oil reservoir. As a method using scanning chemical microscope(SCHEM, pH-Imaging Microscope), the Measurement and estimation of bacterial behavior were carried out in the packed-bed cell made as a sham oil reservoir.In the measurement cell packed full by silica sands which particle size were100-1,000μm, it was could be quantitatively determined the mass transfer of lactic acid using scanning chemical microscope. Next, in the same cell inoculated Lactobacillus casei, the pH values at the cell measured by the microscope. The result was that the pH changed greater at the cell packed full by some smaller size particles. The phenomena were observed at the case using smaller size particle, 45-900μm, for packed-bed cell. On the other hand, in the packed-bed column using some size particles, the amount growth of bacteria was greater as well as the particle size was smaller. In the case of smaller size particle, calculating from the yield factor of the bacteria growth in liquid culture, the bacteria density was increased to 3-5times in comparison with the bigger size. The mathematical model expressed bacterial growth and transport behavior in twodimension packed-bed cell was made, and the phenomena could be analyzed sufficiently by the model.Therefore, by the method in this study, the bacterial growth behavior in packed-bed cell was determined.