Establishment of an analytical method for bacterial behaviors in an oil reservoir.

本工作的目的是建立一种油藏细菌行为的分析方法。作为一种使用扫描化学显微镜(SCEM，pH成像显微镜)的方法，在作为假油藏的填充床池中进行了细菌行为的测量和评价，在用粒度为100~1000μm的硅砂填充的测量池中，利用扫描化学显微镜可以定量地测定乳酸的传质。接下来，在接种干酪乳杆菌的同一细胞中，用显微镜测量细胞的pH值。结果是，当细胞内充满较小尺寸的颗粒时，pH变化较大。这种现象是在填充床电池使用较小尺寸的颗粒(45-900μm)的情况下观察到的。另一方面，在使用一定尺寸颗粒的填充床柱中，细菌的生长数量越大，颗粒越小。在颗粒较小的情况下，根据液体培养中细菌生长的产率因子计算，细菌密度比较大的颗粒提高了3-5倍。建立了描述细菌在二维填充床细胞内生长和传输行为的数学模型，该模型能够充分分析细菌在二维填充床细胞中的生长行为，从而确定了细菌在填充床细胞中的生长行为。

It is Purpose of this work that establishment of an analytical method for bacterial behaviors in an oil reservoir. As a method using scanning chemical microscope(SCHEM, pH-Imaging Microscope), the Measurement and estimation of bacterial behavior were carried out in the packed-bed cell made as a sham oil reservoir.In the measurement cell packed full by silica sands which particle size were100-1,000μm, it was could be quantitatively determined the mass transfer of lactic acid using scanning chemical microscope. Next, in the same cell inoculated Lactobacillus casei, the pH values at the cell measured by the microscope. The result was that the pH changed greater at the cell packed full by some smaller size particles. The phenomena were observed at the case using smaller size particle, 45-900μm, for packed-bed cell. On the other hand, in the packed-bed column using some size particles, the amount growth of bacteria was greater as well as the particle size was smaller. In the case of smaller size particle, calculating from the yield factor of the bacteria growth in liquid culture, the bacteria density was increased to 3-5times in comparison with the bigger size. The mathematical model expressed bacterial growth and transport behavior in twodimension packed-bed cell was made, and the phenomena could be analyzed sufficiently by the model.Therefore, by the method in this study, the bacterial growth behavior in packed-bed cell was determined.