Establishment of an analytical method for bacterial behaviors in an oil reservoir.

建立油藏中细菌行为的分析方法是本研究的目的。采用扫描化学显微镜（扫描电镜、ph成像显微镜）对模拟储层的填充床细胞进行了细菌行为的测量和评价。在粒径为100 ~ 1000 μm的硅砂填充的测量池中，利用扫描化学显微镜可以定量测定乳酸的传质。接下来，在同一细胞中接种干酪乳杆菌，用显微镜测量细胞处的pH值。结果是，当细胞被一些较小的颗粒填充时，pH值变化更大。采用45 ~ 900μm的小颗粒填充床电池，观察到上述现象。另一方面，在使用一定粒径颗粒的填充床柱中，细菌的生长量较大，粒径较小。在颗粒尺寸较小的情况下，从细菌在液体培养中生长的产率因子计算，细菌密度比较大的颗粒增加了3-5倍。建立了二维填充床细胞中细菌生长和转运行为的数学模型，该模型能充分分析细菌生长和转运的现象。因此，通过本研究的方法，测定了细菌在填充床细胞中的生长行为。

It is Purpose of this work that establishment of an analytical method for bacterial behaviors in an oil reservoir. As a method using scanning chemical microscope(SCHEM, pH-Imaging Microscope), the Measurement and estimation of bacterial behavior were carried out in the packed-bed cell made as a sham oil reservoir.In the measurement cell packed full by silica sands which particle size were100-1,000μm, it was could be quantitatively determined the mass transfer of lactic acid using scanning chemical microscope. Next, in the same cell inoculated Lactobacillus casei, the pH values at the cell measured by the microscope. The result was that the pH changed greater at the cell packed full by some smaller size particles. The phenomena were observed at the case using smaller size particle, 45-900μm, for packed-bed cell. On the other hand, in the packed-bed column using some size particles, the amount growth of bacteria was greater as well as the particle size was smaller. In the case of smaller size particle, calculating from the yield factor of the bacteria growth in liquid culture, the bacteria density was increased to 3-5times in comparison with the bigger size. The mathematical model expressed bacterial growth and transport behavior in twodimension packed-bed cell was made, and the phenomena could be analyzed sufficiently by the model.Therefore, by the method in this study, the bacterial growth behavior in packed-bed cell was determined.