Research of Reduction of Productive Inhibition Used Iron-oxidizing Bacteria

本研究旨在减少细菌产生的Fe^<3+>抑制作用。研究结果如下：1.无生长条件下富集培养物中铁氧化细菌的氧化速率随着Fe^<3+>浓度的增加而降低，目前报道的竞争性抑制无法解释这种抑制作用; 2.实验结果表明，从富集培养中分离的氧化亚铁硫杆菌菌株T23-3在纯培养中的生产抑制效果和机理与富集培养相同;用分离自科氏冶炼厂的菌株ATCC 23270和菌株E-15进行试验，结果表明，其产酶抑制效果和机理与富集培养相同;在新的抑制模型中，Fe^3+与铁氧化酶的竞争比为2：1，该模型可以解释ATCC 23270、E-15、T23-3和铁氧化细菌富集培养的结果。Fe^<3+>抑制的机理是相同的，但不同菌株的作用程度不同。因此，根据铁氧化细菌所用Fe^<3+>浓度选择菌株，有可能减轻生产抑制。在选择的情况下，该模型可以是有用的; 6.在无生长条件下，各菌株对铁氧化细菌的生产性抑制机理相同。

This study is investigated to reduce the inhibition of Fe^<3+> produced by the bacteria. The results are as follows ;1. the oxidation rate by the iron-oxidizing bacteria in enrichment culture under the no growth condition reduces with increasing Fe^<3+> concentration, and competitive inhibition reported until now cann't explain this inhibition ; 2. as a result of the experiment used strain T23-3 of Thiobacillus ferrooxidans isolated from enrichment culture, the effect and mechanism of productive inhibition are the same in pure culture as in enrichment culture ; 3. as a result of the experiment used strain ATCC23270 and strain E-15 isolated from Kosaka smelter, the effect and mechanism of productive inhibition are the same as in enrichment culture ; 4. in new inhibition model, Fe^<3+> competes against iron oxidation enzyme 2 to 1, and this model can explain the results of ATCC23270, E-15, T23-3 and the iron-oxidizing bacteria in enrichment culture ; 5. the mechanism of Fe^<3+> inhibition is the same but the degree of the effect is different every strains. So it is possible to reduce productive inhibition, if you select a strain according to Fe^<3+> concentration where the iron-oxidizing bacteria used. And in case of selection the model can be useful ; 6. at every strains the mechanism of productive inhibition of iron-oxidizing bacteria is the same under the no growth condition.