Joint Research on Microbial Enhancement Oil Recovery

本项目的主要成果总结如下：1。在日本和中国吉林省的炼油厂、油藏和油田中发现了许多微生物。这些微生物被分离出来，作为优良的、有用的产气剂、表面活性剂产油剂或原油降解剂。选取阴沟肠杆菌TRC-322、阴沟肠杆菌TU-7A、地衣芽孢杆菌TRC-18-2-a和未确定菌株12#作为候选菌株在吉林油田进行田间试验。在油藏中也鉴定出了相关菌株3。在含4%糖蜜和合成盐水的培养基中，对这些选定的菌株的培养效果最好。此外，在培养基中添加磷酸盐对细菌生长也有促进作用。室内驱油试验表明，微生物对采收率的影响较大。水驱剩余油的最大采收率约为16%。获得了许多以碳氢化合物（主要是正石蜡）为生长底物的分离物。对这些分离菌株的特性进行了研究。通过对吉林石油集团有限责任公司所属各油藏的详细评价，选择扶余油田东部两个区块作为吞吐实验和驱油实验的试验场。在选定的区块进行了12#菌株的吞吐实验，结果表明，采收率比对照提高了约1.5倍。

The main results of this project are summarized as follows1.Many microorganisms were gathered from oil refineries, oil reservoirs and oil fields in Japan and Jilin province, China. These microorganisms were isolated and selected as superior and useful gas producers, surfactant producers or oil degraders.2.Among these isolates, Enterobacter cloacae TRC-322, Enterobacter cloacae TU-7A,Bacillus licheniformis TRC-18-2-a and unidentified strain of 12# were selected as candidate strain for field trial at Jilin oilfield. Also, related strains were identified in the oil reservoir3.The medium inclutded 4% molasses and synthetic brine was most effective for cultivation of these selected isolates. Also, addition of phosphate to the medium was enhanced for bacterial growth.4.Laboratory flooding experiment snowed that microbial effect on oil recovery was high. Maximum recovery of the waterflood residual oil was about l6%.5.Many isolates which utilized hydrocarbon (mainly n-paraffin) as growth substrate were obtained. The characteristic properties of these isolates were investigated.6.Two blocks in the East area of Fuyu oilfield were selccted for the test field of Huff and Puff experiment and flooding experiment after detail evaluation of each oil reservoir belonging to Jilin Petroleum Group Co.Ltd..7.Huff and Puff experiments performed in selected blocks with 12# strain showed that oil recovery was about 1.5-fold greater than that of the control.