Joint Research on Microbial Enhancement Oil Recovery

本项目的主要研究结果如下：1.从日本和中国吉林省的炼油厂、油藏和油田中收集到了大量的微生物。2.筛选出阴沟肠杆菌TRC-322、阴沟肠杆菌TU-7A、地衣芽孢杆菌TRC-18-2-a和未鉴定菌株12#作为吉林油田现场试验的候选菌株。3.以糖蜜浓度为4%的培养基和人工盐水为最适培养条件。在培养基中添加磷酸盐可以促进细菌的生长。4.室内驱油实验表明，微生物对原油采收率的影响很大。水驱残余油的最高采收率为16%左右。5.分离到许多以烃类（主要是正构烷烃）为生长基质的菌株。6.通过对吉林石油集团有限责任公司所属各油藏的详细评价，选择扶余油田东区两个区块作为吞吐实验和驱油实验的试验场。7.用12号菌株在选定区块进行的吞吐实验表明，采收率比对照提高1.5倍左右。

The main results of this project are summarized as follows1.Many microorganisms were gathered from oil refineries, oil reservoirs and oil fields in Japan and Jilin province, China. These microorganisms were isolated and selected as superior and useful gas producers, surfactant producers or oil degraders.2.Among these isolates, Enterobacter cloacae TRC-322, Enterobacter cloacae TU-7A,Bacillus licheniformis TRC-18-2-a and unidentified strain of 12# were selected as candidate strain for field trial at Jilin oilfield. Also, related strains were identified in the oil reservoir3.The medium inclutded 4% molasses and synthetic brine was most effective for cultivation of these selected isolates. Also, addition of phosphate to the medium was enhanced for bacterial growth.4.Laboratory flooding experiment snowed that microbial effect on oil recovery was high. Maximum recovery of the waterflood residual oil was about l6%.5.Many isolates which utilized hydrocarbon (mainly n-paraffin) as growth substrate were obtained. The characteristic properties of these isolates were investigated.6.Two blocks in the East area of Fuyu oilfield were selccted for the test field of Huff and Puff experiment and flooding experiment after detail evaluation of each oil reservoir belonging to Jilin Petroleum Group Co.Ltd..7.Huff and Puff experiments performed in selected blocks with 12# strain showed that oil recovery was about 1.5-fold greater than that of the control.