Improvement of Enrichment Culture Used Iron-oxidizing Bacteria having Inhibition to Cupper

研究了从某矿山废弃物中提取的富集培养物对亚铁离子和元素硫的氧化作用。在含硫培养条件下，测定了Cu ^2+浓度为0、1、10、100、1000 mg·dm ~（-1）时，细菌从S_0开始产生SO_2 ~-的时间变化，以及细菌和原生动物的细胞数<-3>。在含亚铁离子的培养物中，通过在无营养盐条件下或含抑制细菌生长的SDS条件下的铁氧化实验，研究了细菌与亚铁离子的相互作用。在含亚铁离子的培养液中，进一步用高效液相色谱分析了细菌释放的有机物，结果表明：（1）原生动物使细菌氧化速率和硫氧化细菌生长的产率因子比单独培养时小;（2）在Cu^&lt;2+&gt;抑制细菌生长的条件下，原生动物在Cu^&lt;2+&gt;浓度超过100 mg·dm ~（-1）时不生长<-3>，原生动物对Cu^&lt;2+&gt;不产生抗性。（3）在不添加营养盐的情况下，富集培养的铁氧化细菌的氧化速率随培养时间的延长而增加;（4）在SDS浓度大于1 × 10 ~（-1）mol·dm ~（-1）时，铁氧化细菌受到抑制<-5><-3>;（5）在培养液中，HPCL检测到细菌产生的有机物质。

The oxidation of ferrous ion and elemantal sulfur by the enrichment culture collected from a mine waste is investigated. In the culture with sulfur, the time-changes of SO^2-_ produced by the bacteria from S^0 and the cell numbers of the bacteria and the protozoa are measured under the conditions, Cu^<2+> concentration, 0,1,10,100,1000mg dm^<-3>. In the culture with ferrous ion, this study, also, investigated an echosystem with the bacteria through some experiments of iron oxidation in absence of nutritive salts or with SDS that inhibits the growth of the bacteria. In the culture broth with ferrous ion, further, organic materials produced by the bacteria liberated were analyzed by HPCL.The results are as follow ;(1) the protozoa makes the bacterial oxidation rate and the yield factor of sulfur-oxidizing bacteria growth smaller than those in a cultivation of only the bacteria ; (2) while the bacteria grow under the inhibition by Cu^<2+>, the protozoa do not grow at Cu^<2+> concentration above 100mg dm^<-3>, and the resistence to Cu^<2+> does not appear in the protozoa. ; (3) for the absence of nutritive salts, the oxidation rate by the iron-oxidizing bacteria in enrichment culture increases with time ; (4) the iron-oxidizing bacreia is inhibited above 1*10^<-5>mol dm^<-3> in SDS concentration ; (5) organic materials produced by the bacteria are detected by HPCL in the culture broth.