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Molecular patho-biochemical analysis on synovial and discal lesion in the human TMJ

人类颞下颌关节滑膜和椎间盘病变的分子病理生化分析

基本信息

批准号：
09470448
负责人：
SHIBATA Takanori
金额：
$ 8.45万
依托单位：
Yamagata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 2000
项目状态：
已结题

项目摘要

1) Calcified lesions in the Temporomandibular disc were found in 9 of 245 discs(3.7%). In the 5 discs with perforations, calcified lesions were located at the lowersurface of the disc next to the perforation. In the 4 discs without perforations, the lesions were found in the middle layer at the anterior border of the posterior band. In the discs with perforations, lamellae with lacunae and bone marrow were evident. Around the lamellae, chondroid bones were observed. In the discs without perforations that were enclosed by type-2 collagen, chondrocyte-like cells were scattered among the deranged fibers. Hyaline degeneration was also evident. In these discs, the bone was not fully formed. Type-1 collagen was not evident. The present findings suggest that calcification of the human pathological disc occurs by one of 2 mechanisms, ossification orheterocalcification.2)The purpose of this study is to observe the intra- or extracellular particle of breakdown silicone TMJ interpositional implant, SilasticR, and to measure the size of the intracellular particles of them. A specimen was taken from the surrounding tissue of the implanted silicone in the right TMJ of a female patient aged 55 years old, who had the suspected silicon-induced foreign body reaction and lymphadenopathy after TMJ discectomy with SilasticR of 1.3 mm thickness as an interpositional implant, which had been placed three years 11 months before. The samples were submitted for light microscopy and TEM. Two hundred particles were measured with slide calipers on three photographs of TEM. The intracellular particles of breakdown SilasticR, TMJ interpositional implant whose size was 0.471 ± 0.423 (mean±SD) μm, were present adjacent to the implant, and might be associated with inflammation and bone resorption.

1) 245个颞下颌盘有钙化灶9个（3.7%）。在5个有穿孔的椎间盘中，钙化病变位于靠近穿孔的椎间盘下表面。在4个无穿孔的椎间盘中，病变位于后腱束前缘的中间层。有穿孔的椎间盘明显可见带腔隙的椎板和骨髓。骨板周围可见软骨样骨。在没有穿孔的椎间盘中，被2型胶原包围，软骨细胞样细胞散布在紊乱的纤维中。透明样变性也很明显。在这些椎间盘中，骨头没有完全形成。1型胶原不明显。目前的研究结果表明，人类病理椎间盘的钙化是通过骨化或异钙化两种机制之一发生的。2)本研究的目的是观察击穿硅酮TMJ植入物SilasticR的胞内或胞外颗粒，并测量其胞内颗粒的大小。1例55岁女性患者，于3年11个月前置入1.3 mm厚度的SilasticR为间置植入物，经TMJ椎间盘切除术后疑似硅致异物反应及淋巴结病变，取右TMJ植入硅胶周围组织标本。将样品送交光学显微镜和透射电镜。用滑动卡尺在三张透射电镜照片上测量了200个粒子。TMJ植入体的胞内颗粒尺寸为0.471±0.423 (mean±SD) μm，存在于植入体附近，可能与炎症和骨吸收有关。