Molecular patho-biochemical analysis on synovial and discal lesion in the human TMJ

1)245个关节盘中9个(3.7%)有钙化病变。在5个有穿孔的椎间盘中，钙化病变位于穿孔旁的椎间盘下表面。未穿孔的4个椎间盘中，病变位于后束前缘的中层。在穿孔的椎间盘中，有明显的板层，有陷窝和骨髓。板层周围可见软骨样骨。在无穿孔的盘中被2型胶原包裹，软骨细胞样细胞散布在错乱的纤维中。玻璃样变性也很明显。在这些椎间盘中，骨还没有完全形成。1型胶原蛋白表达不明显。2)本研究的目的是观察崩解硅胶TMJ种植体SilasticR的细胞内外颗粒，并测量其细胞内颗粒的大小。取材于3年前11个月前植入1.3 mm厚度SilasticR的TMJ椎间盘摘除术后疑似硅胶异物反应和淋巴结肿大的女性患者右侧TMJ硅胶种植体周围组织标本。样品被提交进行光学显微镜和透射电子显微镜检查。用滑动卡尺在三张电子显微镜照片上测量了200个颗粒。崩解SilasticR的细胞内颗粒大小为0.471±0.423(平均±SD)μm，与种植体相邻，可能与炎症和骨吸收有关。

1) Calcified lesions in the Temporomandibular disc were found in 9 of 245 discs(3.7%). In the 5 discs with perforations, calcified lesions were located at the lowersurface of the disc next to the perforation. In the 4 discs without perforations, the lesions were found in the middle layer at the anterior border of the posterior band. In the discs with perforations, lamellae with lacunae and bone marrow were evident. Around the lamellae, chondroid bones were observed. In the discs without perforations that were enclosed by type-2 collagen, chondrocyte-like cells were scattered among the deranged fibers. Hyaline degeneration was also evident. In these discs, the bone was not fully formed. Type-1 collagen was not evident. The present findings suggest that calcification of the human pathological disc occurs by one of 2 mechanisms, ossification orheterocalcification.2)The purpose of this study is to observe the intra- or extracellular particle of breakdown silicone TMJ interpositional implant, SilasticR, and to measure the size of the intracellular particles of them. A specimen was taken from the surrounding tissue of the implanted silicone in the right TMJ of a female patient aged 55 years old, who had the suspected silicon-induced foreign body reaction and lymphadenopathy after TMJ discectomy with SilasticR of 1.3 mm thickness as an interpositional implant, which had been placed three years 11 months before. The samples were submitted for light microscopy and TEM. Two hundred particles were measured with slide calipers on three photographs of TEM. The intracellular particles of breakdown SilasticR, TMJ interpositional implant whose size was 0.471 ± 0.423 (mean±SD) μm, were present adjacent to the implant, and might be associated with inflammation and bone resorption.