Molecular patho-biochemical analysis on synovial and discal lesion in the human TMJ

1)颞下颌关节盘钙化9例（3.7%）。在5例穿孔的椎间盘中，钙化灶位于穿孔旁的椎间盘下表面。4例无穿孔的椎间盘中，病变位于后带前缘的中层。在有穿孔的椎间盘中，明显可见带陷窝的椎板和骨髓。椎板周围可见软骨样骨。在没有被2型胶原包围的穿孔的椎间盘中，软骨细胞样细胞分散在紊乱的纤维中。透明变性也很明显。在这些椎间盘中，骨没有完全形成。1型胶原不明显。本研究的目的是观察硅橡胶TMJ间置植入物SilasticR的细胞内颗粒和细胞外颗粒，并测量其细胞内颗粒的大小。从一位55岁女性患者的右侧颞下颌关节内植入硅胶的周围组织中采集了一个样本，该患者在颞下颌关节椎间盘切除术后疑似硅引起的异物反应和淋巴结病，其中1.3 mm厚的SilasticR作为间置植入物，该植入物在3年11个月前植入。将样品提交用于光学显微镜和TEM。用游标卡尺在三张TEM照片上测量了200个颗粒。TMJ间置种植体周围存在大小为0.471 ± 0.423（mean±SD）μm的细胞内颗粒，可能与炎症和骨吸收有关。

1) Calcified lesions in the Temporomandibular disc were found in 9 of 245 discs(3.7%). In the 5 discs with perforations, calcified lesions were located at the lowersurface of the disc next to the perforation. In the 4 discs without perforations, the lesions were found in the middle layer at the anterior border of the posterior band. In the discs with perforations, lamellae with lacunae and bone marrow were evident. Around the lamellae, chondroid bones were observed. In the discs without perforations that were enclosed by type-2 collagen, chondrocyte-like cells were scattered among the deranged fibers. Hyaline degeneration was also evident. In these discs, the bone was not fully formed. Type-1 collagen was not evident. The present findings suggest that calcification of the human pathological disc occurs by one of 2 mechanisms, ossification orheterocalcification.2)The purpose of this study is to observe the intra- or extracellular particle of breakdown silicone TMJ interpositional implant, SilasticR, and to measure the size of the intracellular particles of them. A specimen was taken from the surrounding tissue of the implanted silicone in the right TMJ of a female patient aged 55 years old, who had the suspected silicon-induced foreign body reaction and lymphadenopathy after TMJ discectomy with SilasticR of 1.3 mm thickness as an interpositional implant, which had been placed three years 11 months before. The samples were submitted for light microscopy and TEM. Two hundred particles were measured with slide calipers on three photographs of TEM. The intracellular particles of breakdown SilasticR, TMJ interpositional implant whose size was 0.471 ± 0.423 (mean±SD) μm, were present adjacent to the implant, and might be associated with inflammation and bone resorption.