Establishment of the Analytical System on a Fine Volume Synovial Fluid in Human Temporomandibular Joints

1)横断面研究TMJ关节盘病患者关节滑液中硫酸角蛋白(KS)的浓度。55例女性患者62个关节(平均34.1岁，平均29岁)经MRI诊断为关节盘前移位伴(WR；24个关节)或无复位(WOR；24个关节)，并诊断为骨性关节炎(OA；14个关节)，用单抗(1~205D4)电化学发光免疫分析法(ECLIA)测定KS浓度。在62个关节(100%)中，SF内可检出KS。WR组、WOR组和OA组的KS浓度模式和范围分别为1779.4、617.22和387.75和280.32~39495.5、~8740和126.48~3510.5 ng/ml。这一差异在统计学上具有统计学意义(p&lt；0.05)。2)在一项横断面研究中，调查在椎间盘疾病患者囊内冲洗期间回收的样本中TMJ或可检测到的白介素6(IL-6)的频率。14例女性患者共14个关节，年龄16~60岁，平均37.0岁，平均26岁。回收的样品用时间序列采集法在20个14ml试管中分别获得约10ml。每个样品用真空冷冻干燥器浓缩成大约2毫升。采用酶联免疫吸附试验(ELISA)试剂盒扩增亲和素-生物素，两步夹心法测定IL-6浓度。282个关节标本中有12个关节(85.7%)或116个关节标本(41.1%)可检测到IL-6。关节中IL-6阳性率为90.0%~0%(平均41.4%，中位数40%)。检出频率超过85%的3个关节与未检出的2个关节的临床表现不同。

1) To investigate in a cross sectional study the concentrations of keratan sulfate (KS) in the direct aspirated synovial fluid (SF) in patients with TMJ disc dis-orders. Sixty-two joints of 55 female patients (mean 34.1, mode 29 years old) were diagnosed as an anterior displaced disc with (WR; 24 joints) or without reduction (WOR; 24 joints), and with osteoarthritis (OA; 14 joints) by MR imagery.KS concentrations was quantified by electrochemilumine-scence immunoassay (ECLIA) with a monoclonal antibody (1-20 5D4). In 62 joints (100%), KS in SF was detectable. The mode and ranges of KS concentrations in the WR, WOR and OA groups was 1779.4, 617.22 and 387.75, and 280.32-39495.5, 64-8740 and 126.48-3510.5 ng/ml., respectively. This difference among the groups was statistically significant (p<0.05).2) To investigate in a cross sectional study the frequencies of the TMJ or the sample with detectable Interleukin 6 (IL-6) in the recovered samples during the intra capsular irrigation in patients with disc disorders. Fourteen joints of 14 female patients (ranged 16 to 60, mean 37.0 years, mode 26 years) were diagnosed as an anterior displaced disc without reduction by MR imagery. The recovered samples were obtained approximately 10 ml each in the twenty 14ml-test tubes with the time-series collection. Each sample was concentrated into approximately 2 ml with vacuum freeze dryer. The concentration of IL-6 was quantified by enzyme-linked immuno-sorbent assay (ELISA) kit with the avidin-biotin amplified, two step sandwich method. In 12 joints (85.7%) or 116 samples of all 282samples(41.1%), IL-6 was detectable. The frequencies of samples with detectable IL-6 in the joint each were ranged from 90.0% to 0% (mean 41.4%, median 40%). Three joints with over 85% detectable frequency showed the different clinical feature from the undetectable 2 joints.