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Study on molecular architecture and its application

分子结构研究及其应用

基本信息

批准号：
10145102
负责人：
MATSUNAGA Tadashi
金额：
$ 70.85万
依托单位：
Tokyo University of Agriculture and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas (A)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10145102/
关键词：
Molecular archtecture Bacterial magnetic particles Protein A Lipid-tagged antibody lipospme β-Cyclodextrin Surfactant Synthesized sugar lipid molecular architecture bacterial magnetic particle molecular imprinting artificial receptor

项目摘要

In cell surface, many recepter proteins are presented and the specific biomolecules are recognized by these recepter proteins. These mechanisms can be analyzed from chemical view points. To systemize the biotergeting, the molecular architecture of these biomolecules were investigated. Dr. Matsunaga studied molecular architecture using bacterial magnetic particles (BMPs). Successful display of functional proteins such as luciferase, proteinA and receptor on BMP was demonstrated using anchor proteins, MagA or a newly isolated BMP membrane protein Mms16, through gene fusion. ProteinA-displayed BMPs was used for chemiluminescence immunoassay of insulin and high sensitive assay. Furthermore, expression of active receptor on BMPs for estrogen binding assay was also confirmed. A novel approach, in vitro direct insertion, for immobilizing functional proteins on BMPs using the native protein, MagA was developed. Through this technique, active luciferase could be successfully displayed on BMPs. … More Dr. Kobatake studied the displaying method using site-specific lipid-tagged anti-2-phenyl -oxazolone single-chain antibody and lipid-tagged antibody-binding protein were prepared by genetic engineering for displaying antibody molecules on liposome surfaces. The lipid-tagged proteins were expressed in E. coli and translocated at the bacterial membrane. They were stably incorporated into liposomes with high orientation by anchoring the lipid-tail into the hydrophobia layer on the liposome membrane. Dr. Komiyama studied the cross-linkage of β-cyclodextrin (β-CyD) with diisocyante linker and molecular imprinting to the molecule. It was suggested that the molecular imprinting effect is effective in regulating host molecules to cooperatively bind guest molecules. Dr.Goto studied the architecture on the liposome surface. The reaction rate of the surfactant-protease complex was 100 times higher than that of the native one. A biotargeting effect of liposomes that includes the newly synthesized sugar lipid was studied. The degree of targeting effect of liposome was evaluated by the binding test and it was found that the sugar lipid exhibited an excellent bio-targeting effect. Less

在细胞表面，存在许多受体蛋白，这些受体蛋白识别特定的生物分子。这些机制可以从化学角度进行分析。为了使生物接合系统化，研究了这些生物分子的分子结构。Matsunaga博士使用细菌磁性颗粒（BMP）研究分子结构。利用锚蛋白MagA或新分离的BMP膜蛋白Mms 16，通过基因融合，成功地展示了BMP上的功能蛋白，如荧光素酶、蛋白A和受体。将蛋白A展示的骨形成蛋白用于胰岛素的化学发光免疫分析和高灵敏度分析。此外，还证实了用于雌激素结合试验的活性受体在BMP上的表达。开发了一种新的方法，体外直接插入，用于使用天然蛋白MagA将功能蛋白固定在BMP上。通过该技术，可以成功地将活性荧光素酶展示在BMP上。 ...更多信息 Kobatake博士研究了使用位点特异性脂质标记的抗2-苯基恶唑酮单链抗体和脂质标记的抗体结合蛋白的展示方法，通过基因工程制备，用于在脂质体表面展示抗体分子。脂质标记蛋白在E.大肠杆菌中，并易位在细菌膜上。通过将脂质尾锚定到脂质体膜上的疏水层中，它们以高取向稳定地掺入脂质体中。Komiyama博士研究了β-环糊精（β-CyD）与二异氰酸酯连接体的交联和分子印迹。分子印迹效应是调控主体分子与客体分子协同结合的有效机制。后藤博士研究了脂质体表面的结构。该复合物的反应速率比天然复合物高100倍。研究了包含新合成的糖脂质的脂质体的生物靶向作用。通过结合实验评价了脂质体的靶向性，发现糖脂具有良好的生物靶向性。少