Study on molecular architecture and its application

在细胞表面，存在许多受体蛋白，特定的生物分子被这些受体蛋白识别。这些机理可以从化学的角度进行分析。为了使生物靶化系统化，研究了这些生物分子的分子结构。Matsunaga博士利用细菌磁性颗粒（BMPs）研究分子结构。通过基因融合，锚定蛋白、MagA或新分离的BMP膜蛋白Mms16成功地在BMP上展示了荧光素酶、蛋白ina和受体等功能蛋白。蛋白显示的bmp用于胰岛素的化学发光免疫分析和高敏感分析。此外，雌激素结合试验也证实了活性受体在bmp上的表达。开发了一种新的方法，即体外直接插入，利用天然蛋白MagA将功能蛋白固定在bmp上。通过该技术，可以成功地在bmp上显示活性荧光素酶。Kobatake博士研究了使用位点特异性脂质标记的抗2-苯基恶唑酮单链抗体和脂质标记的抗体结合蛋白的显示方法，并通过基因工程制备了用于在脂质体表面显示抗体分子的抗体结合蛋白。脂质标记蛋白在大肠杆菌中表达，并在细菌膜上易位。它们通过将脂尾锚定在脂质体膜的疏水层上，稳定地融入到具有高取向的脂质体中。小宫山博士研究了β-环糊精（β-CyD）与二异氰酸酯连接物的交联以及分子印迹。提示分子印迹效应是调控宿主分子与客体分子协同结合的有效机制。后藤博士研究了脂质体表面的结构。表面活性剂-蛋白酶复合物的反应速率比天然复合物高100倍。研究了含新合成糖脂的脂质体的生物靶向作用。通过结合试验评价脂质体的靶向作用程度，发现糖脂具有良好的生物靶向作用。少

In cell surface, many recepter proteins are presented and the specific biomolecules are recognized by these recepter proteins. These mechanisms can be analyzed from chemical view points. To systemize the biotergeting, the molecular architecture of these biomolecules were investigated. Dr. Matsunaga studied molecular architecture using bacterial magnetic particles (BMPs). Successful display of functional proteins such as luciferase, proteinA and receptor on BMP was demonstrated using anchor proteins, MagA or a newly isolated BMP membrane protein Mms16, through gene fusion. ProteinA-displayed BMPs was used for chemiluminescence immunoassay of insulin and high sensitive assay. Furthermore, expression of active receptor on BMPs for estrogen binding assay was also confirmed. A novel approach, in vitro direct insertion, for immobilizing functional proteins on BMPs using the native protein, MagA was developed. Through this technique, active luciferase could be successfully displayed on BMPs. … More Dr. Kobatake studied the displaying method using site-specific lipid-tagged anti-2-phenyl -oxazolone single-chain antibody and lipid-tagged antibody-binding protein were prepared by genetic engineering for displaying antibody molecules on liposome surfaces. The lipid-tagged proteins were expressed in E. coli and translocated at the bacterial membrane. They were stably incorporated into liposomes with high orientation by anchoring the lipid-tail into the hydrophobia layer on the liposome membrane. Dr. Komiyama studied the cross-linkage of β-cyclodextrin (β-CyD) with diisocyante linker and molecular imprinting to the molecule. It was suggested that the molecular imprinting effect is effective in regulating host molecules to cooperatively bind guest molecules. Dr.Goto studied the architecture on the liposome surface. The reaction rate of the surfactant-protease complex was 100 times higher than that of the native one. A biotargeting effect of liposomes that includes the newly synthesized sugar lipid was studied. The degree of targeting effect of liposome was evaluated by the binding test and it was found that the sugar lipid exhibited an excellent bio-targeting effect. Less