Development of new adsorbents for heavy metals using microorganisms and their microcharacterization.

利用微生物及其微观表征开发新型重金属吸附剂。

• 批准号: 10450393
• 负责人: NAKAJIMA Akira
• 金额: $ 7.42万
• 依托单位: Miyazaki Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10450393/
• 关键词: Uranium; Thorium; Copper; Streptomyces; Arthrobacter; Persimmon tannin; ESR; Hydroxy radical; ESR; ニトロン; 金; 重金属吸着; 微生物; 活性酸素; Anthrobacter; 柿渋タンニン; 樹皮; トリウム; Streptomyces levoris; Arthrobacter nicotianae; 電子スピン共鳴

Microorganisms, such as Streptomyces levoris and Arthrobacter nicotianae, were found to have high abilities to sorb uranium, thorium and copper. Immobilized persimmon tannin has also have high affinities for vanadium and chromium. Thus, both biosorbents could be used for hevy metal recovery and removal from aqueous systems. The ESR study on biosorption gave us that microscopic circumstances, such as pH, around copper in microbial cells affected on the chemical state of copper, and that vanadium should be adsorbed on the immobilized persimmon tannin gels through the reduction process. The rise and fall of activated oxygens, such as hydroxy radical, in metal (Cu(II), V(IV) and U(VI))-H_2O_2 systems, was affected by microbial cells and tannin compounds through their masking and anti-oxidant effects. Thus, these effects could be used for the microcharacterization of the biosorbents. In order to evaluate the heavy metal stress in biosystems, some ESR techniques, such as the ESR on-line measurements using microdialysis and the ESR imaging using spin probes, was established. The interaction between spintraps and heavy metal ions was also examined. These results were publishe as 23 papers in several journals and presented as 33 papers in the international and domestic meetings.

微生物，如左旋链霉菌和烟叶节杆菌，被发现有很强的能力吸收铀、钍和铜。固定化柿子单宁对钒、铬也有较高的亲和力。因此，这两种生物吸附剂都可以用于水系统中的重金属回收和去除。生物吸附的ESR研究告诉我们，微生物细胞中铜周围的微观环境（如pH）会影响铜的化学状态，并且通过还原过程将钒吸附在固定化的柿子单宁凝胶上。金属(Cu(II)， V（IV）和U(VI))-H_2O_2体系中活性氧（如羟基自由基）的上升和下降受微生物细胞和单宁化合物的掩蔽和抗氧化作用的影响。因此，这些效应可以用于生物吸附剂的微观表征。为了评估生物系统中的重金属胁迫，建立了一些ESR技术，如微透析在线测量ESR和自旋探针成像ESR。研究了自旋带与重金属离子的相互作用。这些结果在多个期刊上发表了23篇论文，在国际和国内会议上发表了33篇论文。

项目成果

Yokoyama, H., Lin, Y., Itoh, O., Ueda, Y., Nakajima, A., Ogata, T., Sato, T., Ohya-Nishiguchi, H., Kamada, H.: "EPR imaging for in vivo analysis of the half-life of a nitroxide radical in the hippocampus and cerebral cortex of rats after epileptic seizure

横山，H.，林，Y.，伊藤，O.，上田，Y.，中岛，A.，绪方，T.，佐藤，T.，Ohya-Nishiguchi，H.，镰田，H.：“EPR成像

Ueda, Y., Yokoyama, H., Nakajima, A., Ohya-Nishiguchi, H., Kamada, H.: "In vivo electron spin resonance spectroscopy on signal decay of intrastriatal nitroxide radical after acute administration of haloperiodol in rats."Brain Research Bulletin. 51. 313-31

Ueda, Y.、Yokoyama, H.、Nakajima, A.、Ohya-Nishiguchi, H.、Kamada, H.：“体内电子自旋共振光谱对大鼠急性施用卤代周期醇后纹状体内硝基氧自由基信号衰减的影响。”

Nakajima, A., Sakaguchi, T.: "Uptake and removal of iron by immobilized persimmon tannin."Journal of Chemical Technology and Biotechnology. 75. 977-982 (2000)

Nakajima, A.，Sakaguchi, T.：“固定化柿单宁对铁的吸收和去除。”化学技术与生物技术杂志。

Tokumaru, J., Ueda, Y., Yokoyama, H., Naka-jima, A., Doi, T., Ohya-Nishiguchi, H., Kamada, H.: "In vivo evaluation of anti-oxidant ability of zoni-samide."Proceeding of 3rd International Congress on Neuropsychiatry. 153 (2000)

Tokumaru, J.、Ueda, Y.、Yokoyama, H.、Naka-jima, A.、Doi, T.、Ohya-Nishiguchi, H.、Kamada, H.：“zoni 抗氧化能力的体内评价

Nakajima, A.: "Effect of selenium on Uranium Biosorption."Journal of Radioanalytical and Nuclear Chemistry. 247. 347-350 (2001)

Nakajima, A.：“硒对铀生物吸附的影响。”放射分析与核化学杂志。