Site specific sorting signals for the seed storage protein accumulation

种子储存蛋白积累的位点特异性分选信号

• 批准号: 12138207
• 负责人: TANAKA Kunisuke
• 金额: $ 24.58万
• 依托单位: Kyoto Prefectural University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12138207/
• 关键词: rice; protein body; endosperm cell; prolamin; glutelin; signal peptide; protein transport; cDNA library

We studied the accumulation processes of storage proteins in the developing rice endosperm cells as a model system to understand the general mechanisms for the gene expression, the biosynthesis, and transport of plant storage proteins. The major objectives of this study are, 1) To clarify the differential mechanism of the endosperm cell, 2) To specify the targeting site of storage proteins on the ER membrane, and 3) To characterize the membranes of protein body type II and aleurone particle.As the result of 1), we isolated an Rcdl cDNA that is expressed specifically at the very early developing stage ( at the third day after flowering, starchy endosperm cell and aleurone cell are not yet differentiated).2) Prolamin synthesis was observed on the growing protein body I (PB-I) membrane and the adjacent area to the PB membrane. A Myc antibody was used in this investigation.3) The distribution of the tonoplast intrinsic protein ( TIP ) isoforms on the membrane of protein body II and aleurone particle indicated both organelles derived from vacuoles. The different combinations of the TIP isoforms on both particles indicated the different developmental processes are existing to from these organelles.

我们以水稻胚乳细胞为模型系统，研究了储存蛋白在发育中的积累过程，以了解植物储存蛋白基因表达、生物合成和转运的一般机制。本研究的主要目的是：1)阐明胚乳细胞的分化机制；2)明确内质膜上储存蛋白的靶向位点；3)对II型蛋白体和糊粉颗粒的膜进行表征。通过实验1)，我们分离到了一个在发育早期（开花后第三天，淀粉胚乳细胞和糊粉细胞尚未分化）特异性表达的Rcdl cDNA。2)在生长中的蛋白体I （PB-I）膜及PB膜邻近区域观察到Prolamin合成。本研究使用Myc抗体。3)在蛋白体II和糊粉颗粒的膜上分布的tono质体固有蛋白（TIP）同工异构体表明这两种细胞器都来源于液泡。TIP异构体在两种颗粒上的不同组合表明，这些细胞器存在不同的发育过程。

项目成果

Urano, J.: "Molecular cloning and characterization of a rice dehydroascorbate reductase"FEES Letters. 466. 107-111 (2000)

Urano, J.：“水稻脱氢抗坏血酸还原酶的分子克隆和表征”费用快报。

Furukawa, S.: "Distribution of storage proteins in low-glutelin rice seed determined using a fluorescent antibody."J. of Bioscience and Bioengineering. 96. 467-473 (2003)

Furukawa, S.：“使用荧光抗体测定低谷蛋白水稻种子中储存蛋白的分布。”J.

Okada, A.: "Accumulation of Japanese cedar pollen allergen, Cry j 1, in the protein body I of transgenic rice seeds using the promoter and singal sequence of glutelin GluB-1 gene."Mol. Breed.. 12. 61-70 (2003)

Okada, A.：“利用谷蛋白 GluB-1 基因的启动子和信号序列，在转基因水稻种子的蛋白体 I 中积累日本雪松花粉过敏原 Cry j 1。”

Shigeto Morita: "Air Pollution and Plant Biotechnology [Prospects for Phytomonitoring and phytoremediation], Detoxification of active oxygen species and tolerance in plants exposed to air pollutants and CO_2"Springer-Verlag. (2002)

Shigeto Morita：“空气污染和植物生物技术[植物监测和植物修复的前景]，活性氧的解毒和暴露于空气污染物和CO_2的植物的耐受性”施普林格出版社。

森田重人, 田中國介: "活性酸素消去系の酸化ストレス応答"生吻工学会誌. 79. 311-314 (2001)

Shigeto Morita、Kunisuke Tanaka：“活性氧清除系统的氧化应激反应”《生物造口工程学会杂志》79. 311-314 (2001)。