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Breeding techniques for the content control of physiologically active substances in seed crops

种子作物生理活性物质含量控制育种技术

基本信息

批准号：
07556135
负责人：
TANAKA Kunisuke
金额：
$ 4.1万
依托单位：
Kyoto Prefectural University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

项目摘要

Following results have been obtained through activities of the groups listed below, Activities have been focused on the establishment of breeding techniques for the content control of physiologically active substances in seed crops.・ Biochemical analysis group:1) The analytical accuracy of the isofocusing eletrophoresis for rice allergenic substances has dramatically improved.2) The detection sensitivity for rice subtilicin inhibitor (RASI) and rice trypsin inhibitor (RATI) was improved greatly by using antigen-antibody method.3) The detection technique to detect the existence of the photoperiod-sensitivity cytoplasmic male sterility factor was improved.・ Genetic engineering group:4) The membrane recognizing ability of rice storage protein signal sequences was successfully changed introducing mutations in the sequences.5) Mutations were introduced into the promoter region of a soybean lipoxygenase gene and the promoter activity was changed.6) The direction was fixed to remove allergenic substances from soybean by the isolation of two novelgenes.・ screening Group:7) By use of a new analytical method a line that increased the rutin contents up to 30% was found.8) New soybean varieties were screened they were expected to have good taste.9) Genetic variations of barley amylase inhibitor activities were analyzed using molecular markers.Results described above will be useful for the screening of varieties of physiologically active substances in the seed crops.

通过下列小组的活动取得了以下成果，活动的重点是建立种子作物中生理活性物质含量控制的育种技术。·生化分析组：1）等聚焦电泳法对水稻致敏物质的分析准确性有了显著提高; 2）抗原抗体法对水稻枯草杆菌蛋白酶抑制剂（RASI）和水稻胰蛋白酶抑制剂（RATI）的检测灵敏度有了显著提高; 3）改进了光敏感细胞质雄性不育因子的检测技术。基因工程组：4）通过对水稻贮藏蛋白信号肽序列进行突变，成功地改变了水稻贮藏蛋白信号肽序列的膜识别能力; 5）在大豆脂肪氧合酶基因启动子区进行突变，改变了启动子活性; 6）通过分离两个新基因，确定了去除大豆过敏物质的方向。筛选组：7）采用新的分析方法，筛选出芦丁含量提高30%的品系; 8）筛选出口感优良的大豆新品种; 9）利用分子标记技术，对大麦淀粉酶抑制剂活性的遗传变异进行了分析，为种子作物生理活性物质品种的筛选提供了依据。