Studies of T cell Recognition for Viral Antigen Epitopes

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In the present studies, we demonstrated the 3-dimensional structure of HLA class I・peptide complex recognized by cytotoxic T lymphocytes(CTL) and investigated CTL recognition ex vivo using HLA class I tetramers.(1) We demonstrated the 3-dimensional structure of two HLA-B^*5101-HIV-1 epitope peptide complexes. The structure were compared to those of HLA-B^*3501 and HLA-B^*5301.(2) We established methods to detect antigen-specific CD8^+ T cells using HLA class I tetramers. 11HIV-1 epitopes, one HCV epitope and two HBV epitopes were used to make the tetramers. These tetramers were useful to detect epitope-specific CD8^+ T cells in PBMC from patients infected with HIV, HCV or HBV.(3) We demonstrated increase of HIV-1 specific CD28^-CD45RA^- CD8^+ T cells in PBMC of chronically HIV-1 infected individuals and HCV-specific CD28^+CD45RA^-CD8^+ T cells in PBMC of individuals with acute HCV infection(4) We demonstrated that a single CTL clone recognize a single peptide presented by two different HLA class I, HLA-B^*5101 and HLA-B^*3501.