Development of CTL induced vaccine using reverse immunogenetics.

使用反向免疫遗传学开发 CTL 诱导疫苗。

• 批准号: 06557022
• 负责人: TAKIGUCHI Masafumi
• 金额: $ 6.85万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06557022/
• 关键词: Vaccine; CTL; T cell epitope; HLA class I; peptide; リバース・イムノジェネテックス; HLAクラス工抗原; 細胞障害性T細胞; HLAクラス工抗原結合ペプチド; 抗原提示; リバースイムノジェネテックス法; HLAクラスI抗原; HLAクラスI抗原結合ペプチド

1.We established a binding assay using RMA-S cells transfected with various HLA class I genes in order to test the binding of a panel of peptides to HLA class I molecules. A panel of peptides carrying anchor residues of HLA-B^*3501, B^*5101 and A^*2402 which derived from HIV-1, HCV and HTLV-1 was tested using the binding assay. We identified 170,50 and 131 8-mer to 11-mer peptides which bound to HLA-B^*3501, B^*5101 and A^*2402, respectively.2.We attempted to induce specific CTL in PBL from two HLA-1 infected donors carrying HLA-B^*3501 by 27 HLA-B^*3501 binding peptides. Eleven peptides induced to specific CTL.Ten of these peptides was identified as HIV-1 CTL epitopes presented by HLA-B^*3501 molecules. Nine of these epitopes was further confirmed by generation of the specific CTL clones.3.Identification of both HIV-1 CTL epitopes presented by HLA-B^*5101 or A^*2402 and HCV CTL epitopes presented by HLA-B^*3501 or A^*2402 is under investigation.

1.我们利用转染了不同HLA - I类基因的RMA-S细胞建立了一种结合实验，以测试一组肽与HLA - I类分子的结合。利用结合实验检测了一组携带来自HIV-1、HCV和HTLV-1的HLA-B^*3501、B^*5101和A^*2402锚定残基的肽。我们鉴定出170个、50个和131个8 ~ 11聚肽分别与HLA-B^*3501、B^*5101和A^*2402结合。我们尝试用27个HLA-B^*3501结合肽诱导两个携带HLA-B^*3501的HLA-1感染供者PBL中的特异性CTL。11个肽诱导特异性CTL。其中10个肽被鉴定为HLA-B^*3501分子呈递的HIV-1 CTL表位。其中9个表位通过特异性CTL克隆的产生得到进一步证实。目前正在研究HLA-B^*5101或A^*2402呈递的HIV-1 CTL表位和HLA-B^*3501或A^*2402呈递的HCV CTL表位的鉴定。

项目成果

Yuji Takamiya: "HLA-B^*3501-peptide interactions:Role of anchor residues of peptides in their binding to HLA-B^*3501 molecules." International Immundogy. 6. 255-261 (1994)

Yuji Takamiya：“HLA-B^*3501-肽相互作用：肽的锚定残基在与 HLA-B^*3501 分子结合中的作用。”

John Sideny: "Several HLA alleles overlapping peptide specificities." Journal of Immunology. 154. 247-259 (1995)

John Sideny：“几个 HLA 等位基因与肽特异性重叠。”

Kristen Falk: "Peptide motifs of HLA-B58,B60,B61 and B62 molecules" Immunogenetics. 41. 165-168 (1995)

Kristen Falk：“HLA-B58、B60、B61 和 B62 分子的肽基序”免疫遗传学。

Kristen Falk, Olaf Rotzschke, Masafumi Takiguchi, Volker Gnau, Stefan Stevanovic, Gunter Jung, and Hans-Georg Rammensee: "Peptide motifs of HLA-B38 and B39 molecules." Immunogenetics.41. 162-164 (1995)

Kristen Falk、Olaf Rotzschke、Masafumi Takiguchi、Volker Gnau、Stefan Stevanovic、Gunter Jung 和 Hans-Georg Rammensee：“HLA-B38 和 B39 分子的肽基序。”

Akiko Kikuchi: "Binding of nonamer peplicles to three HLA-B51 hcelecules which differ by a single amino acid substitution in the A-pocket" Immunogenetics. 印刷中

Akiko Kikuchi：“九聚体与三个 HLA-B51 hcelecule 的结合，这三个 HLA-B51 hcelecules 的不同之处在于 A 袋中的单个氨基酸取代”免疫遗传学正在出版。