Development of personal vaccine for virus

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In the present study, we identified multiple HIV-1, HCV and HBV-specific cytotoxic T cell epitopes using reverse immunogengetics.(1) Identification of HLA-A^* 1101-restricted HIV-1 CTL epitopes : We identified three and one CTL epitopes derived from Pol and Env proteins of HIV-1 clade B, respectively. Furthermore, we investigated whether 8 clade B CTL epitopes including the four epitopes are also clade E epitopes. Three were cross-clade epitopes between clade B and E while three clade E-specific sequences corresponding to clade B epitopes were clade E-specific epitopes.(2) Identification of HIV-1 clade B epitopes presented by HLA-A^*3303 : We identified 6 HIV-1 clade B epitopes presented by HLA-A^*3303 using reverse immunogenetics.(3) Identification of HLA-A^*2402-restricted HBV-specific CTL epitopes : We identified two HLA-A^*2402-restricted HBV-specific CTL epitopes using reverse immunogenetics. These epitope-specific CTL were detected in acute phase PBMC from individuals infected with acute HBV hepatitis by specific tetramers.