Development of personal vaccine for virus

开发针对病毒的个人疫苗

• 批准号: 10557034
• 负责人: TAKIGUCHI Masafumi
• 金额: $ 7.49万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10557034/
• 关键词: HLA class I; cytotoxic T lymphocyte; epitope; vaccine; HLA-A11; HIV-1; reverse immunogenetics; HLA-A33; 細胞傷害性T細胞

In the present study, we identified multiple HIV-1, HCV and HBV-specific cytotoxic T cell epitopes using reverse immunogengetics.(1) Identification of HLA-A^* 1101-restricted HIV-1 CTL epitopes : We identified three and one CTL epitopes derived from Pol and Env proteins of HIV-1 clade B, respectively. Furthermore, we investigated whether 8 clade B CTL epitopes including the four epitopes are also clade E epitopes. Three were cross-clade epitopes between clade B and E while three clade E-specific sequences corresponding to clade B epitopes were clade E-specific epitopes.(2) Identification of HIV-1 clade B epitopes presented by HLA-A^*3303 : We identified 6 HIV-1 clade B epitopes presented by HLA-A^*3303 using reverse immunogenetics.(3) Identification of HLA-A^*2402-restricted HBV-specific CTL epitopes : We identified two HLA-A^*2402-restricted HBV-specific CTL epitopes using reverse immunogenetics. These epitope-specific CTL were detected in acute phase PBMC from individuals infected with acute HBV hepatitis by specific tetramers.

在本研究中，我们确定了多个HIV-1，HCV和HBV特异性细胞毒性T细胞表位使用反向免疫遗传学。(1)HLA-A^* 1101限制的HIV-1 CTL表位的鉴定：我们分别鉴定了来自HIV-1进化枝B的Pol和Env蛋白的三个和一个CTL表位。此外，我们研究了包括这四个表位在内的8个进化枝B CTL表位是否也是进化枝E表位。3个是进化枝B和E之间的交叉进化枝表位，而对应于进化枝B表位的3个进化枝E特异性序列是进化枝E特异性表位。(2)HLA-A^*3303呈递的HIV-1进化枝B表位的鉴定：我们使用反向免疫遗传学鉴定了HLA-A^*3303呈递的6个HIV-1进化枝B表位。(3)HLA-A^*2402限制性HBV特异性CTL表位的鉴定：我们使用反向免疫遗传学鉴定了两个HLA-A^*2402限制性HBV特异性CTL表位。这些表位特异性CTL检测急性期PBMC从感染急性HBV肝炎的个人的特异性四聚体。

项目成果

Hiroko Tomiyama: "Identification of Multiple HIV-1 CTL Epitopes Presented by HLA-B^*5101 Molecules"Human Immunology. 60. 177-186 (1999)

Hiroko Tomiyama：“由 HLA-B^*5101 分子呈现的多个 HIV-1 CTL 表位的鉴定”人类免疫学。

Katsuhiko Fukada, Hiroko Tomiyama, Yoshitomo Chujoh, Kiyoshi Miwa, Yutaro Kaneko, Shinichi Oka and Masafumi Takiguchi: "HLA-A^*1101-restricted CTL recognition for a novel epitope derived from the HIV-1 Env protein."AIDS.. 13. 2597-2599 (1999)

Katsuhiko Fukada、Hiroko Tomiyama、Yoshitomo Chujoh、Kiyoshi Miwa、Yutaro Kaneko、Shinichi Oka 和 Masafumi Takiguchi：“HLA-A^*1101 限制性 CTL 识别源自 HIV-1 Env 蛋白的新表位。”AIDS.. 13

Yuji Sobao, Kiyoshi Miwa and Masafumi Takiguchi: "The role of the amino acids associated with the HLA-Bw4/Bw6 epitope in peptide binding to HLA-B molecules."Immunogenetics.. 49. 819-822 (1999)

Yuji Sobao、Kiyoshi Miwa 和​​ Masafumi Takiguchi：“与 HLA-Bw4/Bw6 表位相关的氨基酸在与 HLA-B 分子结合的肽中的作用。”免疫遗传学.. 49. 819-822 (1999)

Masaaki Ibe: "Identification and characterization of a cytotoxic T cell epitope of hepatitis C virus presented by HLA-B^*3501 in acute hepatitis"J.Gen.Virol.. 79. 1735-1744 (1998)

Masaaki Ibe：“急性肝炎中 HLA-B^*3501 呈递的丙型肝炎病毒细胞毒性 T 细胞表位的鉴定和表征”J.Gen.Virol.. 79. 1735-1744 (1998)

Katsuhiko Fukada: "HLA-A^*1101-restricted CTL recognition for a novel epitope derived from the HIV-1 Env protein."AIDS. 13. 2597-2599 (1999)

Katsuhiko Fukada：“HLA-A^*1101 限制 CTL 识别源自 HIV-1 Env 蛋白的新表位。”艾滋病。