Molecular Immunological Analyses of Recognition to T cell For Major and Minor Histocompatibility Antigens

1)CTL克隆对HLA-B_（35）同种异体抗原的识别我们研究了HLA-B_（35）抗原的单个氨基酸替换对HLA-B_（35）特异性CTL克隆识别HLA-B_（35）抗原的影响。结果表明，每个CTL克隆的识别受到不同氨基酸取代的影响，表明对于给定的HLA I类抗原，存在大量同种异体反应性T细胞。我们的研究支持了HLA Ⅰ类分子与不同的同种肽结合形成多个决定簇的观点。2）T细胞克隆对人类次要组织相容性抗原（human minor histocompatibility，hmH）的识别研究了HLA-B35抗原的单个氨基酸取代对hmH特异性T细胞克隆识别hmH抗原的影响。作为肽结合位点的四个残基的取代影响hmH特异性T细胞克隆的识别，表明hmH抗原被识别为肽。事实上，我们成功地从供体来源的B细胞系中分离了hmH肽，但没有从患者来源的B细胞系中分离。

1)Recognition of HLA class I alloantigen by CTL clones.We investigated the effects of single amino acid substitution of HLA-B35 antigen on recognition of HLA-B35 specific CTL clones. The results demonstrated that recognition of each CTL clones are affected by different amino acid substitutions, indicating that there is a large repertoire of alloreactive T cells for a given HLA class I antigen. Our studies support the concepts that multiple determinants are formed by the binding of various allopeptides to the HLA class I molecules.2)Recognition of human minor histocompatibility(hmH)antigen by T cell clones.We investigated the effects of single amino acid substitutions of HLA-B35 antigen on recognition of hmH antigen by hmH specific T cell clones. The substitutions at four residues which are peptide binding sites affects recognition of hmH specific T cell clones, indicating that hmH antigens are recognized as peptides. In fact, we succeeded in isolating the hmH peptide from donor derived B cell lines but not from patient's derived B cell lines.