Molecular Immunological Analyses of Recognition to T cell For Major and Minor Histocompatibility Antigens

1) CTL克隆对HLA I类同种抗原的识别。我们研究了HLA-B35抗原单氨基酸替代对HLA-B35特异性CTL克隆识别的影响。结果表明，对每个CTL克隆的识别受到不同氨基酸替换的影响，这表明存在大量针对给定HLA I类抗原的同种异体反应T细胞库。我们的研究支持这样的概念，即多种同种异体肽与HLA I类分子结合形成了多个决定因子。2) T细胞克隆识别人次要组织相容性（hmH）抗原。我们研究了HLA-B35抗原单氨基酸替换对hmH特异性T细胞克隆识别hmH抗原的影响。作为肽结合位点的四个残基的替换影响了hmH特异性T细胞克隆的识别，表明hmH抗原被识别为肽。事实上，我们成功地从供体来源的B细胞系中分离了hmH肽，但没有从患者来源的B细胞系中分离。

1)Recognition of HLA class I alloantigen by CTL clones.We investigated the effects of single amino acid substitution of HLA-B35 antigen on recognition of HLA-B35 specific CTL clones. The results demonstrated that recognition of each CTL clones are affected by different amino acid substitutions, indicating that there is a large repertoire of alloreactive T cells for a given HLA class I antigen. Our studies support the concepts that multiple determinants are formed by the binding of various allopeptides to the HLA class I molecules.2)Recognition of human minor histocompatibility(hmH)antigen by T cell clones.We investigated the effects of single amino acid substitutions of HLA-B35 antigen on recognition of hmH antigen by hmH specific T cell clones. The substitutions at four residues which are peptide binding sites affects recognition of hmH specific T cell clones, indicating that hmH antigens are recognized as peptides. In fact, we succeeded in isolating the hmH peptide from donor derived B cell lines but not from patient's derived B cell lines.