Study on B cell differentiation signaling by using a novel system

前B细胞受体（preBCR）在B细胞发育早期短暂表达，在B细胞分化中发挥重要作用。 preBCR 传递何种信号来诱导 B 细胞分化仍有待确定。在这项研究中，我们使用我们最初开发的新系统检查了 preBCR 信号转导途径。我们纯化并测序了 36 kD 蛋白 (pp36)，该蛋白通过 preBCR 信号传导进行酪氨酸磷酸化。相应 cDNA 的克隆表明 pp36 是一种在 N 末端区域进行脂质修饰的新型蛋白质。由于已知这种脂质修饰对于蛋白质锚定到脂筏中至关重要，因此 pp36 似乎在信号转导中发挥着重要作用。

Pre B cell receptor (preBCR) is expressed transiently at the early stage of B cell development and plays an important role in B cell differentiation. It remains to be determined what kind of signals are delivered from preBCR to induce B cell differentiation. In this study, we examined the preBCR signal transduction pathway by using a novel system which we have originally developed. We purified and sequenced a 36 kD protein (pp36) that was tyrosine-phosphorylated by preBCR signaling. The cloning of a corresponding cDNA revealed that pp36 is a novel protein with lipid modification at the N-terminal region. Since this lipid modification is know to be essential for proteins to anchor into lipid raft, pp36 appears to play an important role in signal transduction.