Study on B cell differentiation signaling by using a novel system

前B细胞受体（preBCR）在B细胞发育的早期瞬时表达，在B细胞分化中起重要作用。前BCR传递何种信号诱导B细胞分化仍有待确定。在这项研究中，我们研究了前BCR信号转导通路，通过使用一个新的系统，我们最初开发的。我们纯化并测序了一个36 kD的蛋白质（pp 36），这是酪氨酸磷酸化的前BCR信号。相应的cDNA克隆显示，pp 36是一种新的蛋白质与脂质修饰的N-末端区域。由于已知这种脂质修饰对于蛋白质锚入脂筏是必不可少的，因此pp 36似乎在信号转导中起重要作用。

Pre B cell receptor (preBCR) is expressed transiently at the early stage of B cell development and plays an important role in B cell differentiation. It remains to be determined what kind of signals are delivered from preBCR to induce B cell differentiation. In this study, we examined the preBCR signal transduction pathway by using a novel system which we have originally developed. We purified and sequenced a 36 kD protein (pp36) that was tyrosine-phosphorylated by preBCR signaling. The cloning of a corresponding cDNA revealed that pp36 is a novel protein with lipid modification at the N-terminal region. Since this lipid modification is know to be essential for proteins to anchor into lipid raft, pp36 appears to play an important role in signal transduction.