Function and regulation of intermediate filament organization during cell prolifenation and cell signalings.

细胞增殖和细胞信号传导过程中中间丝组织的功能和调节。

• 批准号: 10480205
• 负责人: INAGAKI Masaki
• 金额: $ 6.59万
• 依托单位: Aichi Cancer Center
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10480205/
• 关键词: Phosphorylation; Cleavage furrow; Phosphopeptide antibodies; Rho; Cytokinesis; Intermediate filament (IF); Rho-associated kinase (Rho-kinase); Rho-キナーゼ; ケラチン結合蛋白質; 中間径フィラメント構築制御; 細胞周期; 中間径フィラメント(1F)蛋白質; リン酸化反応; リン酸化ペプチド抗体; Rho

Intermediate filaments (IFs), which form the structural framework of cytoskeleton, have been found to be dramatically reorganized during mitosis. Some protein kinases activated in mitosis are thought to control spatial and temporal IF reorganization through phosphorylation of IF proteins. Rho-associated kinase (Rho-kinase), one of. the putative targets of the small GTPase Rho, does phosphorylate IF proteins, specifically at the cleavage furrow during cytokinesis. This cleavage furrow-specific phosphorylation plays an important role in the local IF breakdown and efficient separation of IF networks, Recent studies on Rho signaling pathways have allowed new models about the molecular mechanism of rearrangements of cytoskeletons including IFs during cytokinesis.

形成细胞骨架结构框架的中间丝（IF）被发现在有丝分裂过程中发生显着重组。有丝分裂中激活的一些蛋白激酶被认为通过 IF 蛋白的磷酸化来控制 IF 的空间和时间重组。 Rho相关激酶（Rho-kinase）之一。小 GTP 酶 Rho 的假定靶标确实会磷酸化 IF 蛋白，特别是在胞质分裂过程中的裂解沟处。这种裂解沟特异性磷酸化在局部 IF 分解和 IF 网络的有效分离中发挥着重要作用。最近对 Rho 信号通路的研究为胞质分裂过程中包括 IF 在内的细胞骨架重排的分子机制提供了新的模型。

项目成果

H.Kosako: "Specific Accumulation of Rho-associated kinase at the Cheavage Funow during cytokinesisi Cleauage furrow-spocific phesphory lation of intermediate filament." Oncogene. (in press).

H.Kosako：“在胞质分裂过程中，Rho 相关激酶在 Cheavage Funow 上的特定积累，中间丝的 Cleauage 沟特异性磷酸化。”

Inagaki, N., Nishizawa, M., Minakata, Y., Yamamoto, H., Takeuchi, Y., Miyamoto, E., Kaibuchi, K., and Inagaki, M.: "Activation of Ca^<2+>/calmodulin-dependent protein kinase llwithin post-synaptic dendritic spines of cultured hippocampal neurons."J.Biol.

Inagaki, N.、Nishizawa, M.、Minakata, Y.、Yamamoto, H.、Takeuchi, Y.、Miyamoto, E.、Kaibuchi, K. 和 Inagaki, M.：“Ca^<2 >/ 的激活”

H.Goto: "Phosphorylation of vimentin by Rho-associated Kinase at a uhigue amino-termnal site that is specifically phosphorylated during cytokinesis" J.Biol.Chem.273. 11728-11736 (1998)

H.Goto：“Rho 相关激酶在胞质分裂过程中特异磷酸化的 uhigue 氨基末端位点对波形蛋白进行磷酸化”J.Biol.Chem.273。

Izawa, I., Nishizawa, M., Ohtakara, K., Ohtsuka, K., Inada, H., and Inaqaki, M.: "Identification of Mrj, a DnaJ/Heat shock protein 40family protein, as akeratin 8/18 filament-regulatory protein."J.Biol. Chem. 275. 34521-34527 (2000)

Izawa, I.、Nishizawa, M.、Ohtakara, K.、Ohtsuka, K.、Inada, H. 和 Inaqaki, M.：“Mrj（一种 DnaJ/热休克蛋白 40 家族蛋白）作为角蛋白 8/18 的鉴定

Goto, H. et. al.: "Identification of a novel phosphorylation site on histone H3 coupled with mitotic chromosome condensation"J. Biol. Chem.. 274. 25543-25549 (1999)

后藤，H.等。