A basic research for the improvement of the biocompatibility of titanium using bone proteins.

利用骨蛋白改善钛生物相容性的基础研究。

• 批准号: 10480246
• 负责人: AYUKAWA Yasunori
• 金额: $ 6.78万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10480246/
• 关键词: titanium; bone; osteoblast; osteocalcin; osteopontin; osseointegration; collagen; ハイドロキシアパタイト; インプラント; 界面

In order to detect the distribution of bone proteins in the newly formted bone around the titanium implant, immunocytochemistry of collagen type I, III (ICol, IIICol), osteopontin (Opn), and osteocalcin (Ocl) was performed toward the bone around the titanium at the day 7, 14 ancl 28 after the installation.At day 7, fine fibrillar layer was seen between the amorphous layer and the bone, in which IIICol, Ocl and Opn were predominantly observed. In the amorphous layer, only Ocl and Opn could be seen. ICol was restricted into the bone matrix and could not be seen in both amorphous layer and the fine fibrillar layer.At day 28, fine fibrillar layer could not be seen anywhere and in consequence no IIICol reaction could be seen on the specimen. In the meantime, ICol-contained bone matrix was seen directly beneath the amorphous layer.

为了检测钛种植体周围新形成骨中骨蛋白的分布，在安装后第7、14、28天对钛种植体周围骨进行I型胶原、III型胶原（ICol、IIICol）、骨桥蛋白（Opn）、骨钙蛋白（Ocl）的免疫细胞化学检测。第7天，在无定形层与骨之间可见细纤维层，其中以IIICol、Ocl和Opn为主。在非晶层中，只能看到Ocl和Opn。ICol被限制在骨基质中，在无定形层和细纤维层均未见ICol。在第28天，任何地方都看不到细小的纤维层，因此在标本上看不到IIICol反应。同时，在非晶层正下方可见含有icol的骨基质。