Production of Bifidogenic Growth Stimulator and Its Application to Development of Probiotics

双歧生长刺激剂的制备及其在益生菌开发中的应用

• 批准号: 10555286
• 负责人: TANIGUCHI Masayuki
• 金额: $ 8.13万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10555286/
• 关键词: Propionibacterium; Bifidobacterium; Co-culture; Biorecactor; Growth factor; Probiotics

1. The concentration and the productivity per time of bifidogenic growth stimulator were increased by using a co-culture system for a homolactic acid bacterium and a propionic acid bacterium, a culture system with microfiltration, a fed-batch culture system with feed back control of carbon source concentration, and a culture system with microfiltration and feed back control of carbon source concentration.2. A novel bioreactor system with two fermentors and microfiltration modules was developed for efficient production bifidobacterial cells by a co-culture of Propionibacterium freudenreichii and Bifidobacterium adolescentis as well as clarification of interactions between the two bacteria.3. The proposed co-culture system allowed the successful exchange of culture medium between two fermentors. When P.freudenreichii and B.adolescentis, B.longum, or B.breve were cultivated individually in the new co-culture system, the amount of bifidobacterial cells produced was 1.2-1.6 fold as high as that with batch culture.

1.采用高乳酸菌和丙酸菌共培养体系、微滤培养体系、碳源浓度反馈控制流加培养体系和微滤反馈控制培养体系，提高了双歧生长促进剂的浓度和单产。通过对弗氏丙酸杆菌和青春型双歧杆菌的共培养，明确了两种细菌之间的相互作用，开发了一种新型的生物反应器系统，该系统由两个发酵罐和微滤组件组成，可以高效地生产双歧杆菌细胞。建议的共培养系统允许两个发酵罐之间成功地交换培养基。在新的混合培养体系中，分别培养青春型、长型和短型双歧杆菌时，双歧杆菌产量是分批培养的1.2-1.6倍。

项目成果

谷口正之: "微生物間相互作用の解析と混合培養システムの開発"化学工学論文集. 25. 149-157 (1999)

Masayuki Taniguchi：“微生物相互作用分析和混合培养系统的开发”化学工程杂志 25. 149-157 (1999)。

Masayuki Taniguchi: "Clarification of Interactions among Microorganisms and Development of Co-culture system"Kagaku Kogaku Ronbunshu. Vol.25, No.2. 149-157 (1999)

Masayuki Taniguchi：“微生物之间相互作用的澄清和共培养系统的开发”Kagaku Kogaku Ronbunshu。

谷口正之: "微生物間相互作用の解析と混合培養システムの開発"化学工学論文集. 25・2. 149-157 (1999)

谷口雅之：“微生物相互作用的分析和混合培养系统的开发”化学工程学报25・2（1999）。