Production of Bifidogenic Growth Stimulator and Its Application to Development of Probiotics

1.采用高乳酸菌和丙酸菌共培养体系、微滤培养体系、碳源浓度反馈控制流加培养体系和微滤反馈控制培养体系，提高了双歧生长促进剂的浓度和单产。通过对弗氏丙酸杆菌和青春型双歧杆菌的共培养，明确了两种细菌之间的相互作用，开发了一种新型的生物反应器系统，该系统由两个发酵罐和微滤组件组成，可以高效地生产双歧杆菌细胞。建议的共培养系统允许两个发酵罐之间成功地交换培养基。在新的混合培养体系中，分别培养青春型、长型和短型双歧杆菌时，双歧杆菌产量是分批培养的1.2-1.6倍。

1. The concentration and the productivity per time of bifidogenic growth stimulator were increased by using a co-culture system for a homolactic acid bacterium and a propionic acid bacterium, a culture system with microfiltration, a fed-batch culture system with feed back control of carbon source concentration, and a culture system with microfiltration and feed back control of carbon source concentration.2. A novel bioreactor system with two fermentors and microfiltration modules was developed for efficient production bifidobacterial cells by a co-culture of Propionibacterium freudenreichii and Bifidobacterium adolescentis as well as clarification of interactions between the two bacteria.3. The proposed co-culture system allowed the successful exchange of culture medium between two fermentors. When P.freudenreichii and B.adolescentis, B.longum, or B.breve were cultivated individually in the new co-culture system, the amount of bifidobacterial cells produced was 1.2-1.6 fold as high as that with batch culture.