Conversion of Agricultural Wastes to Lactic Acid by a Combination of Pentose-fermentative Lactic Acid Bacteria and Reversibly Soluble-insoluble Enzymes

通过戊糖发酵乳酸菌和可逆可溶-不溶酶的组合将农业废物转化为乳酸

• 批准号: 13836003
• 负责人: TANIGUCHI Masayuki
• 金额: $ 2.3万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13836003/
• 关键词: Xylose; L-Lactic acid; Lactic acid bacteria; Agricultural waste; Biomass; Rice bran

1) Glucose and xylose were mainly obtained from delignified rice straw by hydrolysis using cellulase preparation. Glucose and arabinose were mainly obtained from defatted rice bran by hydrolysis using amylase and cellulase preparations.2) Lactobacillus vaccinostericus, Lactobacillus pentosus, Pediococcus pentosaceus, and Enterococcus casseliflavus were selected as a xylose-fermented lactic acid bacterium. More lactic acid was produced from MRS medium containing xylose by E. casseliflavus as compared with other lactic acid bacteria.3) The amount of lactic acid produced did not increase in a fermentation using MRS medium containing xylose and glucose as a carbon source and E. casseliflavus.4) The maintenance of pH at a low value in a simultaneous saccharification and fermentation of non-sterilized rice bran allowed the selective growth of Lactobacillus rhamnosus, a lactic acid bacterium added. About 30 g/L of L-lactic acid was produced from defatted rice bran in a simultaneous saccharification and fermentation in which the pH was kept at 4.5.

1)利用纤维素酶对稻草脱木素后进行水解，主要得到葡萄糖和木糖。主要利用淀粉酶和纤维素酶制剂水解脱脂米糠获得葡萄糖和阿拉伯糖。2）选择牛痘乳杆菌、戊糖乳杆菌、戊糖片球菌和蛋黄肠球菌作为木糖发酵乳酸菌。在含有木糖的MRS培养基中，E. 3）在使用含有木糖和葡萄糖作为碳源的MRS培养基和E. 4）在未灭菌米糠的同时糖化和发酵中将pH维持在低值允许添加的乳酸菌鼠李糖乳杆菌（Lactobacillus rhamnosus）的选择性生长。在pH保持在4.5的同时糖化和发酵中，从脱脂米糠生产约30 g/L的L-乳酸。