Evolution of functions of sperm W chromosome

精子W染色体功能的进化

• 批准号: 10556063
• 负责人: SHIMADA Kiyoshi
• 金额: $ 7.81万
• 依托单位: NAGOYA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10556063/
• 关键词: mouse oocyte; chicken sperm; intracytoplasmic sperm injection (ICSI); sex -reversed chicken; female pronucleus; male pronucleus; W-chromosome; fertility; ニワトリ; 精子; 卵細胞質内精子注入法; W性染色体; ハムスター; 性分化; アロマターゼインヒビター; 性転換; 性染色体; W精子; FISH法; 性決定

We demonstrated that injection of aromatase inhibitor, Fadrozole, (AI) at day 3-5 days of incubation caused sex reversal from female to male chickens. Normal males produce only sperm carrying the Z chromosome (Z sperm), but the sex -reversed hens produce sperm carrying the Z chromosome and W chromosome (W sperm). The presence of W sperm in testes was demonstrated by Southern blot analysis and fluorescence in situ hybridization (FISH). We found that approximately 60% of spermatids were those carrying W chromosome and that about 50% of spermatozoa contained the W chromozome out of 35 spermatozoa examined. To confirm whether the W sperm is functional and fertilizes the oocyte, we conducted intracytoplasmic chicken sperm injection (ICSI) into the hamster ovum. A single sperm was injected into a single hamster ovum. Total of 48 ova injected were incubated at 37 C for 24 hours. According to microscopic observation, 44 (92%) ova were viable and 30 oocytes showed two pronuclei, indicating that injected sperm are capable if inducing at least the early events in zygote formation. These novel findings might ultimately lead to the development of a procedure that produces exclusively female offspring.

我们证明了注射芳香酶抑制剂Fadrozole，（AI）在孵育的第3-5天后导致性逆转从雌性鸡到雄性鸡。正常雄性仅产生携带Z染色体（Z精子）的精子，但性逆转的母鸡产生的精子携带Z染色体和W染色体（W Sperm）。通过Southern印迹分析和荧光原位杂交（FISH）证明了睾丸中W的存在。我们发现，大约60％的精子是携带W染色体的那些精子，大约50％的精子中包含35个精子中的W染色体。为了确认w精子是否具有功能并施肥卵母细胞，我们对仓鼠卵子进行了囊肿内鸡精子注射（ICSI）。将一个精子注入单个仓鼠卵子中。将注射的48个OVA在37 C下孵育24小时。根据显微镜观察，44（92％）的OVA是可行的，30个卵母细胞显示了两个原始核，表明注射精子能够至少诱导合子形成的早期事件。这些新颖的发现最终可能导致制定仅产生女性后代的程序。

项目成果

Ieda T.,Saito N.,and Shimada K.: "Effect of low calcium diet on messenger ribonucleic acid levels of calbindin-D28K of intestine and shell gland in laying hens in relation to egg shell quality."Japanese Poultry Science. 36. 295-303 (1999)

Ieda T.、Saito N.和Shimada K.：“低钙饮食对蛋鸡肠和壳腺钙结合蛋白-D28K 信使核糖核酸水平与蛋壳质量的影响。”《日本家禽科学》。

Furuse M.,Ao R.,Bungo T.,Ando R.,Shimojo M.,Masuda Y.and Saito N.: "Central gastrin inhibits feeding behavior and food passage in neonatal chicks."Life Sciences. 65. 305-311 (1999)

Furuse M.、Ao R.、Bungo T.、Ando R.、Shimojo M.、Masuda Y. 和 Saito N.：“中枢胃泌素抑制新生雏鸡的摄食行为和食物通道。”生命科学。

Furuse M., Matsumoto M., Pinotoan R., Saito N., Sugahara K.and Hasegawa S.: "Intraventricular injection of glucagon-like peptide-1 inhibits water intake of the neonatal chick."Japanese Poultry Science. 36. 229-235 (1999)

Furuse M.、Matsumoto M.、Pinotoan R.、Saito N.、Sugahara K. 和 Hasekawa S.：“脑室内注射胰高血糖素样肽-1 抑制新生小鸡的水摄入。”日本家禽科学。

Bungo T.: "Effects of naloxone on the anorexic action by glucagon-like peptide-1(7-36) in the neonatal chick."Japanese Poultry Science. 36. 109-115 (1999)

Bungo T.：“纳洛酮对新生小鸡胰高血糖素样肽-1(7-36) 厌食作用的影响。”日本家禽科学。

Kita K., Hangsanet K., Shibata T., Conlon M.A., Sasaki T., Saito N.and Okumura J.: "Refeeding increases hepatic insuline-like growth factor-I (IGF-I) gene expression and plasma IGF-I concentration in fasted chicks."British Poultry Science. 39. 679-682 (19

Kita K.、Hangsanet K.、Shibata T.、Conlon M.A.、Sasaki T.、Saito N.和 Okumura J.：“再喂食可增加肝脏胰岛素样生长因子-I (IGF-I) 基因表达和血浆 IGF-I