Studies of molecular mechanisms on the sexual determination and differentiation

性别决定和分化的分子机制研究

• 批准号: 10660268
• 负责人: SHIMADA Kiyoshi
• 金额: $ 2.18万
• 依托单位: NAGOYA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660268/
• 关键词: chicken; estradiol; sexual differentiation; aromatase; anti-Mullerian hormone; in situ hybridization; aromatase inhibitor; in situ hybridization; PCR; アンドロジェンレセプター; 生殖腺; 抗ミュラー管ホルモン; アロマターゼmRNA; インシチュ・ハイブリタイセーション; 性腺

The sex of birds is determined genetically. The bird with homogametic sex (ZZ) is male and has the testis, and the bird with heterogametic sex (ZW) is female and has the ovary. However, there are many unknown mechanisms about the gene expression in related with sexual determination and differentiation. We examined the gene expression of anti-Mullerian hormone (AMH), P450 cl17hydroxylase (P450c17) and P450aromatase (P450arom) in the gonads in chick embryo by in situ hybridization. Although, AMH mRNA was not detected in female gonad. from day 5 of incubation and increased with development, AMH mRNA was not detected in female gonad. P450c17 mRNA was detected in male and female gonads from day 5 of incubation. Although, P450arom mRNA was detected in the female gonad from day 5 of incubation and increased with development, P450arom mRNA was not detected in male gonad. When the female crick was injected with aromatase inhibitor at day 3 of incubation, the level P450arom mRNA was inhibited and the level of AMH mRNA was enhanced. When the male chick was injected with estradiol at day 3 of incubation, the level of AMH mRNA was inhibited and the level of P450arom m RNA was enhanced. These results suggested that AMH and P450arom may be a rival relation for sexual differentiation, and AMH has an important role for male and P450arom has an important role for female.

鸟类的性别是由基因决定的。同配性别的鸟（ZZ）为雄性，有睾丸;异配性别的鸟（ZW）为雌性，有卵巢。然而，与性决定和性分化相关的基因表达机制还不清楚。应用原位杂交技术研究了抗苗勒管激素（AMH）、P450 c17羟化酶（P450 c17）和P450芳香化酶（P450 arom）基因在鸡胚性腺中的表达。在雌性性腺中未检测到AMH mRNA。从孵化第5天开始，AMH mRNA在雌性性腺中未检测到，并随发育而增加。P450 c17 mRNA在孵化第5天开始在雄性和雌性性腺中检测到。P450 arom mRNA在雌性性腺中的表达从孵化第5天开始就开始，并随着发育而增加，但在雄性性腺中没有检测到P450 arom mRNA。在孵育第3天注射芳香化酶抑制剂后，P450 arom mRNA水平受到抑制，AMH mRNA水平受到增强。在孵育第3天注射雌二醇后，AMH mRNA水平被抑制，P450 arom mRNA水平被增强。这些结果提示AMH和P450 arom在性分化中可能存在竞争关系，AMH对雄性起重要作用，P450 arom对雌性起重要作用。

项目成果

Abinawanto: "Identification of sperm bearing female-specific chromosome in the sex-reversed chicken"Journal of Experimental Zoology. 280. 65-72 (1998)

Abinawanto：“性别逆转鸡中携带雌性特异性染色体的精子的鉴定”实验动物学杂志。

Bungo T.: "Effects of naloxone on the anorexic action by glucagon-like peptide-1 (7-36) in the neonatal chick"Japanese Poulty Science. 36. 109-115 (1999)

Bungo T.：“纳洛酮对新生小鸡胰高血糖素样肽-1 (7-36) 厌食作用的影响”日本家禽科学。

Kansaku N.: "Expression of messenger ribonucleic acids of luteinizing hormone receptor,P450 17α-hydroxylase and P450 aromatase in isolated grnulosa,theca interna and theca externa layers of chicken ovarian follicles during follicular growth"Animal Science

Kansaku N.：“卵泡生长过程中鸡卵巢卵泡分离颗粒、内膜层和外膜层中黄体生成素受体、P450 17α-羟化酶和 P450 芳香酶信使核糖核酸的表达”动物科学

Nishikimi H.: "Sex differentiation and mRNA expression of P450c17,P450arom and AMH in gonades of the chicken"Molecular Reproduction and Development. 55. 20-30 (2000)

Nishikimi H.：“鸡性腺中 P450c17、P450arom 和 AMH 的性别分化和 mRNA 表达”分子生殖和发育。

Suzuki T.: "Comparative FISH mapping on Z chromosomes of chicken and Japanese quail"Cytogenetics Cell Genet.. 87. 22-26 (1999)

Suzuki T.：“鸡和日本鹌鹑 Z 染色体上的比较 FISH 作图”《细胞遗传学》Cell Genet.. 87. 22-26 (1999)