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Coordinating mechanisms of prostaglandins and vasotocin in the control of ovulation-oviposition

前列腺素和催产素控制排卵产卵的协调机制

基本信息

批准号：
61560301
负责人：
SHIMADA Kiyoshi
金额：
$ 1.28万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

Uterine electromyographic activity (EMG),plasma concentrations of prostaglandin(PG) F and E, and arginine vasotocin(AVT) were simultaneously measured in relation to PGE_2-induced premature oviposition and to indomethacin-blocked oviposition in chickens. The administration of indomethacin (5 mg, im) 3 hr before the predicted time of oviposition delayed egg lay by 8-14 hr, whereas control hens laid eggs at the predicted time. In indomethacin-treated hens increases in uterine EMG activity and plasma concentrations of PGF,PGE,and AVT were not observed at the expected time of oviposition, whereas a marked increase in plasma PGF and AVT occurred during spontaneous oviposition . The intrauterine administration of PGE_2(1<micrn>g) 3 hr before predicted oviposition induced premature egg lay within 5 min. Premature oviposition was accompanied by significant elevations in both uterine EMG activity and plasma levels of AVT. Changes in plasma levels of PGF and PGE were not observed. When premature oviposition was induced, significant increases in EMG activity and plasma concentrations of PGF and AVT were observed at the expected time of oviposition in the absence of an egg in the uterus. The administration of PGE_2 at the predicted time of oviposition in hens pretreated with indomethacin induced oviposition which was accompanied by a significant increase in plasma AVT levels. The results suggest that AVT release is stimulated by uterine contractility in chickens.

本文同时测定了子宫肌电活动（EMG）、血浆前列腺素（PG）F和E及精氨酸催产素（AVT）浓度与PGE_2诱导的鸡早产和吲哚美辛阻断的鸡排卵的关系。在预测的产卵时间前3小时给予吲哚美辛（5 mg，im），推迟了8-14小时的产蛋，而对照母鸡在预测的时间产蛋。在吲哚美辛处理的母鸡子宫肌电活动和血浆中的PGF，PGE，和AVT的浓度增加，没有观察到在预期的产卵时间，而血浆中的PGF和AVT的显着增加发生在自发产卵。在<micrn>预测产卵前3小时宫内注射PGE_2（1g）可在5分钟内诱导早产卵。早产卵伴随着子宫肌电活动和血浆AVT水平的显著升高。未观察到血浆PGF和PGE水平的变化。当诱导过早产卵时，在子宫中没有卵的情况下，在预期的产卵时间观察到EMG活性和血浆PGF和AVT浓度的显着增加。用消炎痛预处理母鸡，在预测产卵时间给药PGE_2可诱导产卵，并伴有血浆AVT水平显著升高。结果表明，AVT的释放是由鸡子宫收缩刺激的。