Study on functions of Dmrt-1 gene on Z sex chromosome in birds

鸟类Z性染色体Dmrt-1基因的功能研究

• 批准号: 13660286
• 负责人: SHIMADA Kiyoshi
• 金额: $ 1.92万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660286/
• 关键词: Birds; Z sex chromosome; Dmrt-1; male determining factor; testis; Amh; Sox 9; Sertoli cells; 性決定; Z染色体; ニワトリ; whole mount in situ hybridization; mRNA; アンチセンスオリゴヌクレオチド

Dmrt-1 is named after the DM domain for doublesex (dsx) of Drosophila and mab-3 of Caenorhabidtis elegans. Both encode proteins with a novel DNA binding motif called the DM domain. Chicken Dmrt-1 gene on the Z chromosome was first reported in reference to synteny aspect between chicken and human by Nanda et al. (1999). In humans, Dmrt-1 is located within a minimal region of chromosome 9p24.3 deleted in patients with male-to-female sex reversal. It is expressed in the genital ridge and Mullerian duct prior to sexual differentiation at higher levels in ZZ than in ZW embryos of the chicken. Furthermore, this is expressed as early as 3-4 days of incubation far in advance to Amh expression that occurs on day 5 of incubation in male. Interestingly, it is also expressed in the Mullerian ducts which Amh acts in male-specific way. In addition to this sex-and time-specific expression, localization of this gene on the Z chromosome make it an especially good candidate to be involved in avian sex determination.

DMRT-1以果蝇的Doubesex（DSX）和Caenorhabidtis Elegrans的MAB-3的DM域命名。两者都用一种称为DM结构域的新型DNA结合基序编码蛋白质。 Nanda等人首先报道了Z染色体上的鸡DMRT-1基因。 （1999）。在人类中，DMRT-1位于男性到女性性逆转患者中删除的9p24.3染色体的最小区域内。它在ZZ的性别分化之前在性别分化之前在生殖器脊和穆勒式管道中表达，而不是在鸡肉的ZW胚胎中。此外，这早在雄性孵育第5天就早于3-4天的孵育3-4天表示。有趣的是，它也以AMH为男性特定方式的穆勒式管道表达。除了这种性别和特定时间的表达外，该基因在Z染色体上的定位使其成为参与禽类性别确定的特别好的候选者。

项目成果

Sakimura, M., Hanzawa, S., Shimada, K: "Effect of estradiol and nonylphenol on mRNA levels of vitellogenin II in the liver of chicken embryos"J.Poult.Scl.,. 38・3. 250-257 (2001)

Sakimura, M., Hanzawa, S., Shimada, K：“雌二醇和壬基酚对鸡胚胎肝脏中卵黄蛋白原 II 的 mRNA 水平的影响”J.Poult.Scl. 38・3。 2001）

Kansaku N., Shimada K.: "Molecular cloning of chicken vasoactive polypeptide receptor"Biology of Reproduction. 64. 575-581 (2001)

Kansaku N.，Shimada K.：“鸡血管活性多肽受体的分子克隆”生殖生物学。

Sakimura M., Usami M., Hanzawa S., Tsukada A., Saito N., Ohno Y., Shimada K.: "Suppression effect of p-nonylphenol on male-specific mRNA expression in the embryonic gonad of chickens"J.Poult.Sci.. 39. 91-99 (2002)

Sakimura M.、Usami M.、Hanzawa S.、Tsukada A.、Saito N.、Ohno Y.、Shimada K.：“对壬基酚对鸡胚胎性腺中雄性特异性 mRNA 表达的抑制作用”J.

Saito N., Sasaki T., Furuse M., Arakawa K., Shimada K.: "Effects of AVT antisense oligodeoxynucleotides on AVT release induced"Comparative Biochemistry and Physiology. 128A. 147-153 (2001)

Saito N.、Sasaki T.、Furuse M.、Arakawa K.、Shimada K.：“AVT 反义寡脱氧核苷酸对 AVT 释放诱导的影响”比较生物化学和生理学。

Sakimura M., Tsukada A., Usami M., Hanzawa S., Saito N., Ohno Y., Shimada K.: "Effect of estradiol and nonylphenol on mRNA expression of estrogen receptors a and b, and cytochrome P450 aromatase in the gonad of chicken embryos, J."Poult,Sci.. 39. 302-309

Sakimura M.、Tsukada A.、Usami M.、Hanzawa S.、Saito N.、Ohno Y.、Shimada K.：“雌二醇和壬基酚对雌激素受体 a 和 b 以及细胞色素 P450 芳香酶 mRNA 表达的影响