Conformational Analysis of Periodic Polypeptides Containing Pro Residue

含Pro残基的周期性多肽的构象分析

• 批准号: 11450373
• 负责人: HAYASHI Toshio
• 金额: $ 8.19万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11450373/
• 关键词: periodic polypeptide; proline; conformation; molecular mechanics; peptide synthesis; circular dicroism; helix; biomaterials; 分子力学計算; 二次構造; β-ヘリックス; 周期性タンパク質; 合成ポリペプチド; 三次元構造解析; ヘリックス構造; 核磁気共鳴スペクトル; 円偏光二色性; 赤外吸収スペクトル; 反復アミノ酸配列

Periodic polypeptides such as poly(Xaa-Pro-Pro-Pro), poly(Xaa-Pro-Pro), poly(Xaa-Pro), poly(Xaa-Xaa-Pro), poly(Xaa-Xaa-Xaa-Pro), (Xaa=Ala, Leu, Val, Phe Glu, Asp, Lys, Arg) were synthesized, and their conformations were analyzed by experimental and theoretical method. It was shown that they take new helical conformations which are clearly distinguished from the well-known secondary structures such as α-helix, β-strand, and polyproline-II structures. It was also shown that relative stabilities of those conformations are changed by solvents and temperature. The experimental results were supported by theoretical analysis using molecular-mechanics calculations.

合成了周期性多肽poly（Xaa-Pro-Pro-Pro）、poly（Xaa-Pro-Pro）、poly（Xaa-Pro）、poly（Xaa-Xaa-Pro）、poly（Xaa-Xaa-Xaa-Pro），（Xaa=Ala、Leu、瓦尔、Phe、Glu、Asp、Lys、Arg），并通过实验和理论方法对其构象进行了分析。结果表明，它们具有新的螺旋构象，这些构象与众所周知的二级结构如α-螺旋、β-链和聚脯氨酸-II结构明显不同。溶剂和温度的变化改变了这些构象的相对稳定性。实验结果得到了分子力学计算理论分析的支持。

项目成果

M.Oka: "Theoretical Analysis of α-Hairpin Structures Stabilized by a Disulfide-Linkage between Two Helices."Peptide Scince.. 1998. 365-368 (1999)

M.Oka：“通过两个螺旋之间的二硫键稳定的 α-发夹结构的理论分析。”肽科学.. 1998. 365-368 (1999)

M.Oka et al.: "Conformational Analysis of Periodic Polypeptides Composed of Proline and Alanine Residues"Biomedical Material.Res.Asia. 5. 548-549 (2001)

M.Oka 等人：“由脯氨酸和丙氨酸残基组成的周期性多肽的构象分析”Biomedical Material.Res.Asia。

T.Kiyota: "Structure and Activity Relationship of D-Amino Acid-Invorporated Antimicrobial Model Polypeptides"Peptide Scince. 1998. 241-244 (1999)

T.Kiyota：“D-氨基酸掺入的抗菌模型多肽的结构和活性关系”肽科学。

M.Wakahara, T.Nakamura, M.Oka, T.Hayashi, M.Hattori, Y.Hirano: "Conformational Analysis of Model Polypeptides Having Repetitive Xaa-Pro-Pro Sequences"Peptide Science. 2001. 289-292 (2002)

M.Wakahara、T.Nakamura、M.Oka、T.Hayashi、M.Hattori、Y.Hirano：“具有重复 Xaa-Pro-Pro 序列的模型多肽的构象分析”肽科学。

T.Nakamura et al.: "Conformational Analysis of Model Polypeptide Having Repetitive Ala-Pro-Pro-Pro Sequence"Peptide Science. 2000. 321-324 (2001)

T.Nakamura 等人：“具有重复 Ala-Pro-Pro-Pro 序列的模型多肽的构象分析”肽科学。