Studies and functions of indolamine N-acetyltransferase in the brain, midgut and reproduction glands in insects

昆虫脑、中肠和生殖腺吲哚胺 N-乙酰转移酶的研究和功能

• 批准号: 11460021
• 负责人: TAKEDA Makio
• 金额: $ 3.9万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11460021/
• 关键词: Neurotransmitter; Biological Clock; Indolamine Metabolism; N-acetyltransferase; Midgut; Reproductive Glands; Photoperiodism; Melatonin; インドールアミン; セロトニン; ワモンゴキブリ; 細胞増殖; 生殖腺成熟; N-アセチル転移酵素(NAT); Cyc; 概日振動; Cycle; Period; Double time; CYC /

cDNAs encoding N-acetyltransferase (NAT) were cloned from Priplaneta americana and Antheraea pernyi. Northern analyses and real time PCR analyses showed that transcriptional activity has day / night periodicity. Immunocytochemical investigations suggest that NAT is closely associated with the circadian clock elements in Bombyx mori and A. pernyi. It seems likely that NAT constitutes an important output regulator in the clock system. Also demonstrated are that NAT functions as a switch regulating cell turn-over rate in the midgut and that it regulates the maturation of oocytes in the ovary. One of the NAT-metabolites, melatonin affected the synchronization of circadian rhythm of locomotion in a cricket and fat accumulation in a bug.

从美洲原蝇和柞蚕中克隆了编码 N-乙酰转移酶 (NAT) 的 cDNA。 Northern分析和实时PCR分析表明转录活性具有昼/夜周期性。免疫细胞化学研究表明，NAT 与家蚕和柞蚕的生物钟元件密切相关。 NAT 似乎构成了时钟系统中重要的输出调节器。还证明了 NAT 作为调节中肠细胞周转率的开关，并调节卵巢中卵母细胞的成熟。褪黑激素是 NAT 代谢物之一，它影响蟋蟀运动的昼夜节律和昆虫脂肪积累的同步。

项目成果

Hironobu Asano: Comparative Biochemistry and Physiology. (in press).

浅野博信：比较生物化学和生理学。

Loeb,M.J.: "Apoptosis in cultured midgut cells from Heliothis virescence larvae exposed to various conditions"Archives of Insect Biochemistry and Physiology. 45. 12-23 (2000)

Loeb，M.J.：“暴露于各种条件下的夜蛾幼虫培养的中肠细胞的凋亡”昆虫生物化学和生理学档案。

Takeda, M.: "Photoperiodic system in physiological reality"Entomological Science. Vol.2 No.4. 567-574 (1999)

Takeda, M.：“生理现实中的光周期系统”昆虫学。

Niva, C.: "Effect of photoperiod and melatonin on nymphal development, polyphenism and reuroduction in Hyalompmha halys (Heteroptera ; Pentatomidae)"Zoological Science. (in press).

Niva, C.：“光周期和褪黑激素对 Hyalompmha halys（异翅目；蝽科）若虫发育、多型性和再生的影响”《动物学科学》。

竹田 真木生: "昆虫学大事典"朝倉書店. 1220 (2003)

武田真纪夫：《昆虫学百科全书》朝仓书店 1220 (2003)。