Molecular mechanisms controlling circadian rhythms and photoperiodism in insects

控制昆虫昼夜节律和光周期的分子机制

• 批准号: 18380043
• 负责人: TAKEDA Makio
• 金额: $ 11.8万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18380043/
• 关键词: 光周性; 概日振動; メラトニン合成系; 光受容; 変態; 休眠; 表現型多型; N-acetyltransferease (NAT); メラトニン; long wave opsin; action sp[ectra; 卵休眠; Cycle; 概日時計; NAT; serotonin; 5HT receptor; UVB; opsin; マダラスズ; pars intercerebralis; 摂食; セロトニン; N-アセチル転移酵素(NAT); 神経分泌

Photoperiodism is an important biological mechanism and involved in agriculture production but its molecular mechanism remains obscure.This study analyzed the structures, localization and dynamics of the elements involved in photoreception, circadian oscillation and endocrine switch to reconstruct the photoperiodic system. Comparative studies using different insects revealed a considerable flexibility in the circadian pacemaker structure and photoreceptor regarding to ontogenetic stages and phylogenetic lines. The important factor mediating photpoperiodic system seems to be the indolaminergic pathway, since the upstream regulatory region of N-acetyltransferase(NAT) that catalyze the production of melatonin has several E-boxes where transcription factors CLK/CYK binds, the cells secreting prothoracicotropic horone (PTTH) express melatonin receptor and all PER containing cells have NAT, and melatonin content and NAT transcript level show a circadian oscillation. These cells have the extraretinal photoreception.

光周期是一种重要的生物学机制，与农业生产密切相关，但其分子机制尚不清楚，本研究从光感受、昼夜节律振荡和内分泌开关等方面分析了光周期系统的结构、定位和动力学，以期重建光周期系统。使用不同的昆虫的比较研究发现，在昼夜节律起搏器的结构和感光器方面的个体发育阶段和系统发育线相当大的灵活性。吲哚胺能途径是光周期系统的重要调节因子，因为催化褪黑素产生的N-乙酰转移酶（NAT）上游调控区有多个与转录因子CLK/CYK结合的E-box，分泌促前胸激素激素（PTTH）的细胞表达褪黑素受体，所有含PER的细胞都有NAT，褪黑素含量和NAT转录水平呈现昼夜振荡。这些细胞具有视网膜外光感受性。

项目成果

Casein kinase I in the Silkworm, Bombyx mori : Structure and possible roles in circadian timing and development

家蚕中的酪蛋白激酶 I：结构及其在昼夜节律和发育中的可能作用

• 发表时间: 2006
• 期刊: J. Biol. Rhythms 21
• 作者: Le Thi Dieu T;Sehadova H.;Ichihara N.,Iwai S. Mita K;Takeda M.
• 通讯作者: Takeda M.

Takeda M. (2009)Putative regulatory mechanism of prothoracicotropic hormone (PTTH) secretion in the American cockroach, Periplaneta americana as inferred from co-locatization of Rab8, PTTH and protein kinase C in neurosectretory cells.

Takeda M. (2009) 根据神经分泌细胞中 Rab8、PTTH 和蛋白激酶 C 的共定位推断美洲大蠊 (Periplaneta americana) 促促胸腺激素 (PTTH) 分泌的假定调节机制。

• 发表时间: 2009
• 期刊: Cell and Tissue Research 335
• 作者: Hiragaki;S.;Uno T.;Takeda M.
• 通讯作者: Takeda M.

Co-localization and unique distributions of two clock proteins CYCLE and CLOCK in the cephalic ganglia of the ground cricket

两种时钟蛋白 CYCLE 和 CLOCK 在地蟋蟀头神经节中的共定位和独特分布

• 发表时间: 2008
• 期刊: Allonemobius allardi Cell and Tissue Research 331
• 作者: Shao;Q-M;Hiragaki;S;Takeda;M
• 通讯作者: M

Molecular characterization and distribution of cycle protein from Athalia rosae

玫瑰花循环蛋白的分子特征和分布

• 发表时间: 2007
• 期刊: J. Insect Physiol. 53
• 作者: Bembenek J;Itokawa K;Hiragaki S.;ShaoQ.M;Tufail M.;Takeda M.
• 通讯作者: Takeda M.

Action spectrum for the suppression of arylalkylamine N-acetyltransferase activity in the two-spotted spider mite Tetranychus urticae Photochemistry and

抑制二斑叶螨芳基烷基胺 N-乙酰转移酶活性的作用谱光化学与

• 发表时间: 2009
• 期刊: Photobiology (in press)
• 作者: Suzuki;T.;N. Izawa;T. Takashima;M. Watanabe;M. Taked
• 通讯作者: M. Taked