Visualization of the site of phospholipid biosynthesis in Arabidopsis roots under low temperature conditions

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WS, cct2, cct1-1and cct1-1 cct2 plants exhibited CCT activity in descending order. cct1-1 cct2 seedlings were unable to elongate roots at 2. Root epidermal cells showed ER-Tracker fluorescence comparably in WS, cct2 and cct1-1 seedlings at ambient temperature and expandedly at 2 or under salt stresses. In contrast, endomembrane structures developed less expensively in cct1-1 cct2 seedlings at ambient temperature and disappeared in cold-or salt-stressed seedlings. sGFP-CCT1 and sGFP-CCT2 fluorescence was associated with ER-Tracker fluorescence in transgenic Arabidopsis roots. These results suggest that functional CCTs are required for stress-induced ER development and cell elongation in Arabidopsis roots.