Reproduction structure of an old-growth evergreen broad-leaved forest analyzed by microsatellite marke

微卫星标记分析古老常绿阔叶林的繁殖结构

• 批准号: 11460069
• 负责人: YAMAMOTO Shin-ichi
• 金额: $ 7.81万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11460069/
• 关键词: Evergreen broad-leaved forest; Old-growth forest; <Castanopsis>___________- <cuspidata>_________-; Heterozygosiry; Microsatellite; Genetic marker; DNA; Forest dynamics; ツバキ

Structure and dynamics of old-growth, evergreen broad-leaved forest community and tree populations were studied in the Tatera Forest Reserve using demographic and molecular biological methods. The population structure was different between co-dominant species, Castanopsis cuspidate var. sieboldii and Distylium racemosum. The spatial distribution pattern of major tree species seemed to be determined rather biological factors such as inter/intra-species competition than environmental factors such as canopy gap and topography. Canopy and tree community dynamics parameters were in balance, indicating this forest being in dynamic-equilibrium. Mortality and recruitment of trees depend on canopy gap characteristics, resulting in that canopy dynamics originated from natural disturbances influence tree composition and community dynamics. The microsatellite markers, which can detect the genetic structure, gene flow and be useful tools for paternity analysis, were developed in Camellia japonica a … More nd C. cuspidata var. sieboldii. For C. japonica, four microsatellite markers were developed. C. japonica were clumped in the stand and weak genetic structure were detected. There was strong genetic structure over short-distance between individuals. Limited seed dispersal and heterogeneity of microsite of stand may influence spatial pattern and genetic structure of C. japonica. For C. cuspidata var. sieboldii, six newly developed microsatellite markers and two markers which were already developed in Quercus species showed high polymorphism and can be useful for reproduction ecology. Sprout regeneration from damaged canopy stem seemed to be important for C. cuspidata var. sieboldii in spite of large amount of saplings. Thus, the spatial structure and dynamics of forest tree community were detected using demographic methods and extend the knowledge of the genetic structure and reproduction ecology for major tree species in evergreen broad-leaved forest using molecular biological methods. I Less

采用种群统计学和分子生物学方法，对塔特拉森林保护区万年青阔叶林群落和乔木种群的结构和动态进行了研究。共优势种的种群结构不同。sieboldii和总状毛萼藓Distyelongracemosum。主要树种的空间分布格局似乎更多地取决于种间/种内竞争等生物学因素，而不是林窗和地形等环境因素。林冠动态参数和乔木群落动态参数处于平衡状态，表明该林处于动态平衡状态。树木的死亡和补充取决于林窗的特征，从而导致源于自然干扰的林冠动态影响树木组成和群落动态。利用微卫星标记技术，对山茶属植物的遗传结构和基因流进行了研究，并对山茶属植物的亲缘关系进行了分析。 ...更多信息 和C.尖形变种西氏菌梭粳稻中，开发了4个微卫星标记。C.粳稻在林分中呈聚集分布，遗传结构较弱。短距离内个体间存在较强的遗传结构。有限的种子扩散和林分微立地的异质性可能影响杉木的空间格局和遗传结构。日本。梭尖形变种sieboldii、6个新开发的微卫星标记和2个已在栎属物种中开发的微卫星标记显示了较高的多态性，可用于生殖生态学研究。从受损的冠层茎段再生植株对柑橘来说是重要的。尖形变种尽管有大量的树苗，但仍有大量的sieboldii。因此，本研究利用种群统计学方法对森林树木群落的空间结构和动态进行了研究，并利用分子生物学方法对万年青阔叶林主要树种的遗传结构和繁殖生态学进行了研究。一减

项目成果

Nishimura, N., Hara, T., Miura, M., Manabe, T. and Yamamoto, S.: "Tree competition and species coexistence in a warm-temperate old-growth evergreen broad-leaved forest in Japan"Plant Ecology. (accepted for publication).

Nishimura, N.、Hara, T.、Miura, M.、Manabe, T. 和 Yamamoto, S.：“日本暖温带古老常绿阔叶林中的树木竞争和物种共存”植物生态学。

Miura, M., Manabe, T., Nishimura, N., Yamamoto, S.: "Forest canopy and community dynamics in a temperate old-growth evergreen broad-leaved forest, SW Japan a 7-year study of a 4-ha plot."Journal of Ecology. 89. 841-849 (2001)

Miura, M.、Manabe, T.、Nishimura, N.、Yamamoto, S.：“日本西南部温带古老常绿阔叶林的森林冠层和群落动态，一项对 4 公顷土地进行的为期 7 年的研究

Ueno, S., Tomaru, N., Yoshimaru, H., Manabe, T. and Yamamoto, S.: "Genetic structure of Camellia japonica L. in an old-growth evergreen forest, Tsushima, Japan"Molecular Ecology. 9. 647-656 (2000)

Ueno, S.、Tomaru, N.、Yoshimar, H.、Manabe, T. 和 Yamamoto, S.：“日本对马岛古老常绿森林中山茶花的遗传结构”分子生态学。

Miura, M., Manabe, T., Nishimura, N. and Yamamoto, S.: "Forest canopy and community dynamics in a temperate old-growth evergreen broad-leaved forest, SW Japan : a 7-year study of a 4-ha plot"Journal of Ecology. 89. 841-849 (2001)

Miura, M.、Manabe, T.、Nishimura, N. 和 Yamamoto, S.：“日本西南部温带古老常绿阔叶林的森林冠层和群落动态：对 4-

Manabe, T., Nishimura, N., Miura, M. and Yamamoto, S.: "Population structure and spatial patterns of trees in a temperate old-growth evergreen broad-leaved forest in Japan"Plant Ecology. 151. 181-197 (2000)

Manabe，T.，Nishimura，N.，Miura，M.和Yamamoto，S.：“日本温带古老常绿阔叶林中树木的种群结构和空间格局”植物生态学。