Reproduction structure of an old-growth evergreen broad-leaved forest analyzed by microsatellite marke

采用人口统计学和分子生物学方法研究了塔塔拉森林保护区常绿阔叶林群落和乔木种群的结构和动态。共优势种三叶栲和总状菊的种群结构存在差异。主要树种的空间分布格局与其说是由林隙和地形等环境因素决定，不如说是由种内竞争等生物因素决定。林冠动态参数与乔木群落动态参数处于平衡状态，表明该森林处于动态平衡状态。树木的死亡和补充依赖于林冠间隙特征，导致自然干扰引起的林冠动态影响树木组成和群落动态。建立了山茶和东北山茶的微卫星标记，可以检测山茶和东北山茶的遗传结构、基因流和父系分析。对于粳稻，开发了4个微卫星标记。粳稻在林分呈块状分布，遗传结构较弱。个体间距离近，遗传结构强。种子传播的有限性和林分微生境的异质性可能影响粳稻的空间格局和遗传结构。新发现的6个微卫星标记和2个已在栎属物种中发现的微卫星标记多态性较高，可用于繁殖生态学研究。尽管三叶草树苗数量庞大，但受损冠层茎的发芽再生似乎对三叶草有重要意义。利用人口统计学方法检测了常绿阔叶林树种的空间结构和动态，并利用分子生物学方法扩展了对常绿阔叶林主要树种遗传结构和繁殖生态学的认识。我少

Structure and dynamics of old-growth, evergreen broad-leaved forest community and tree populations were studied in the Tatera Forest Reserve using demographic and molecular biological methods. The population structure was different between co-dominant species, Castanopsis cuspidate var. sieboldii and Distylium racemosum. The spatial distribution pattern of major tree species seemed to be determined rather biological factors such as inter/intra-species competition than environmental factors such as canopy gap and topography. Canopy and tree community dynamics parameters were in balance, indicating this forest being in dynamic-equilibrium. Mortality and recruitment of trees depend on canopy gap characteristics, resulting in that canopy dynamics originated from natural disturbances influence tree composition and community dynamics. The microsatellite markers, which can detect the genetic structure, gene flow and be useful tools for paternity analysis, were developed in Camellia japonica a … More nd C. cuspidata var. sieboldii. For C. japonica, four microsatellite markers were developed. C. japonica were clumped in the stand and weak genetic structure were detected. There was strong genetic structure over short-distance between individuals. Limited seed dispersal and heterogeneity of microsite of stand may influence spatial pattern and genetic structure of C. japonica. For C. cuspidata var. sieboldii, six newly developed microsatellite markers and two markers which were already developed in Quercus species showed high polymorphism and can be useful for reproduction ecology. Sprout regeneration from damaged canopy stem seemed to be important for C. cuspidata var. sieboldii in spite of large amount of saplings. Thus, the spatial structure and dynamics of forest tree community were detected using demographic methods and extend the knowledge of the genetic structure and reproduction ecology for major tree species in evergreen broad-leaved forest using molecular biological methods. I Less